General Information


DRAVP ID  DRAVPe00044

Peptide Name   NL6-5

Sequence  YFLLKL

Sequence Length  6

UniProt ID  No entry found

Source  Synthetic construct(derived from HIV-1 integrase)



Activity Information


Target Organism  HIV

Assay  Integrase Assay

Activity 

  • [Ref.16854053]HIV-1:Inhibition of 3′-processing catalyzed by integrase(IC50=20 µM);inhibition of strand transfer catalyzed by integrase(IC50=20 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  Integrase

Mechanism  HIV-1 integrase (IN) is essential for viral replication. Following reverse transcription of the RNA into DNA by HIV-1 reverse transcriptase, IN integrates the viral DNA into the host genome.The peptide could inbibit integrase(By inhibiting the two steps of IN catalysis: 3′-processing and strand transfer).



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00044

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C42H65N7O8

Absent amino acids  ACDEGHIMNPQRSTVW

Common amino acids  L

Mass  796.02

Pl  8.59

Basic residues  1

Acidic residues  0

Hydrophobic residues  4

Net charge  1

Boman Index  1205

Hydrophobicity  150

Aliphatic Index  195

Half Life 

  •     Mammalian:2.8 hour
  •     Yeast:10 min
  •     E.coli:2 min

Extinction Coefficient cystines  1490

Absorbance 280nm  298

Polar residues  1



Literature Information


Literature 1

Title   Sequence-based design and discovery of peptide inhibitors of HIV-1 integrase: insight into the binding mode of the enzyme.

Pubmed ID   16854053

Reference   J Med Chem. 2006 Jul 27;49(15):4477-86.

Author   Li HY, Zawahir Z, Song LD, Long YQ, Neamati N.

DOI   10.1021/jm060307u