General Information


DRAVP ID  DRAVPe00173

Peptide Name   P8

Sequence  GTKALTAVIPLTEEAEC

Sequence Length  17

UniProt ID  No entry found

Source  Synthetic construct(derived from Pep-A)



Activity Information


Target Organism  HIV

Assay  RT-Polymerase Assay

Activity 

  • [Ref.18952602]HIV-1:inhibition of polymerase activity of HIV-1 RT(Ki =57 ± 19 μM);inhibition of PHA-P-activated PBMCs infected with HIV-1-LAI(EC50=290 nM,associated with Pep-1).
  • NOTE:Ki: inhibition constants

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  Reverse Transcriptase

Mechanism  HIV-1 reverse transcriptase (RT) plays an essential multifunctional role in the replication of the virus, by catalyzing the synthesis of double-stranded DNA from the single strand retroviral RNA genome.The peptide could inhibit the activity of reverse transcriptase.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00173

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C75H128N18O27S

Absent amino acids  DFHMNQRSWY

Common amino acids  AET

Mass  1746.01

Pl  4.25

Basic residues  1

Acidic residues  3

Hydrophobic residues  7

Net charge  -2

Boman Index  -724

Hydrophobicity  33.53

Aliphatic Index  103.53

Half Life 

  •     Mammalian:30 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  5



Literature Information


Literature 1

Title   A new generation of peptide-based inhibitors targeting HIV-1 reverse transcriptase conformational flexibility.

Pubmed ID   18952602

Reference   J Biol Chem. 2009 Jan 2;284(1):254-264.

Author   Agopian A, Gros E, Aldrian-Herrada G, Bosquet N, Clayette P, Divita G.

DOI   10.1074/jbc.M802199200