General Information

General Information

DRAVP ID DRAVPe00190

Peptide Name PAW

Sequence GTKWLTEWIPLTAEAEC

Sequence Length 17

UniProt ID No entry found

Source Synthetic construct(derived from Pep-A)

Activity Information

Target Organism HIV

Assay RT-Polymerase Assay

Activity

[Ref.18952602]HIV-1:inhibition of polymerase activity of HIV-1 RT(Ki =0.7 ± 0.2 μM);inhibition of PHA-P-activated PBMCs infected with HIV-1-LAI(EC50=1.8 nM,associated with Pep-1).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target Reverse Transcriptase

Mechanism HIV-1 reverse transcriptase (RT) plays an essential multifunctional role in the replication of the virus, by catalyzing the synthesis of double-stranded DNA from the single strand retroviral RNA genome.The peptide could inhibit the activity of reverse transcriptase.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00190

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C89H134N20O27S

Absent amino acids DFHMNQRSVY

Common amino acids ET

Mass 1948.22

Pl 4.25

Basic residues 1

Acidic residues 3

Hydrophobic residues 7

Net charge -2

Boman Index -843

Hydrophobicity -12.35

Aliphatic Index 80.59

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 11000

Absorbance 280nm 687.5

Polar residues 5

Literature Information

Literature 1

Title A new generation of peptide-based inhibitors targeting HIV-1 reverse transcriptase conformational flexibility.

Pubmed ID 18952602

Reference J Biol Chem. 2009 Jan 2;284(1):254-264.

Author Agopian A, Gros E, Aldrian-Herrada G, Bosquet N, Clayette P, Divita G.

DOI 10.1074/jbc.M802199200