General Information

General Information

DRAVP ID DRAVPe00257

Peptide Name 2429

Sequence TTWEEWDREINEYTSRIESLIRESQEQQEKNEQELREL

Sequence Length 38

UniProt ID No entry found

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay MAGI/cMAGI infectivity assay

Activity

[Ref.17640899]HIV IIIB:inhibition of virus infection on CEM4 cells(IC50=0.012 μg/ml);
HIV 098:inhibition of virus infection on CEM4 cells(IC50=0.021μg/ml);
HIV 098-T20:inhibition of virus infection on CEM4 cells(IC50=0.056 μg/ml);
HIV 098-T1249:inhibition of virus infection on CEM4 cells(IC50=0.037 μg/ml);
HIV 098-T651:inhibition of virus infection on CEM4 cells(IC50=0.167 μg/ml).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target membrane

Mechanism The peptide acts by binding to the heptad repeat 1 (HR1) region of gp41 and preventing the interaction of the HR1 and HR2 domains, which is required for virus–cell fusion.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00257

Linear/Cyclic Linear

N-terminal Modification Acetylation

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C205H319N59O76

Absent amino acids ACFGHMPV

Common amino acids E

Mass 4826.14

Pl 4.29

Basic residues 5

Acidic residues 12

Hydrophobic residues 8

Net charge -7

Boman Index -16817

Hydrophobicity -177.89

Aliphatic Index 61.58

Half Life

Mammalian:7.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 12490

Absorbance 280nm 337.57

Polar residues 9

Literature Information

Literature 1

Title Design of helical, oligomeric HIV-1 fusion inhibitor peptides with potent activity against enfuvirtide-resistant virus.

Pubmed ID 17640899

Reference Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12772-7.

Author Dwyer JJ, Wilson KL, Davison DK, Freel SA, Seedorff JE, Wring SA, Tvermoes NA, Matthews TJ, Greenberg ML, Delmedico MK.

DOI 10.1073/pnas.0701478104