General Information


DRAVP ID  DRAVPe00260

Peptide Name   2544

Sequence  MTWEAWDRAIAEYAARIEALIRAAQEQQEKNEAALREL

Sequence Length  38

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  HIV

Assay  MAGI/cMAGI infectivity assay

Activity 

  • [Ref.17640899]HIV IIIB:inhibition of virus infection on CEM4 cells(IC50=0.007 μg/ml);
  • HIV 098:inhibition of virus infection on CEM4 cells(IC50=0.026μg/ml);
  • HIV 098-T20:inhibition of virus infection on CEM4 cells(IC50=0.033 μg/ml);
  • HIV 098-T1249:inhibition of virus infection on CEM4 cells(IC50=0.014 μg/ml);
  • HIV 098-T651:inhibition of virus infection on CEM4 cells(IC50=0.021 μg/ml).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  membrane

Mechanism  The peptide acts by binding to the heptad repeat 1 (HR1) region of gp41 and preventing the interaction of the HR1 and HR2 domains, which is required for virus–cell fusion.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00260

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C194H307N57O61S

Absent amino acids  CFGHPSV

Common amino acids  A

Mass  4445.98

Pl  4.64

Basic residues  5

Acidic residues  8

Hydrophobic residues  18

Net charge  -3

Boman Index  -9296

Hydrophobicity  -60.26

Aliphatic Index  87.89

Half Life 

  •     Mammalian:30 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  12490

Absorbance 280nm  337.57

Polar residues  3



Literature Information


Literature 1

Title   Design of helical, oligomeric HIV-1 fusion inhibitor peptides with potent activity against enfuvirtide-resistant virus.

Pubmed ID   17640899

Reference   Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12772-7. 

Author   Dwyer JJ, Wilson KL, Davison DK, Freel SA, Seedorff JE, Wring SA, Tvermoes NA, Matthews TJ, Greenberg ML, Delmedico MK.

DOI   10.1073/pnas.0701478104