General Information

General Information

DRAVP ID DRAVPe00278

Peptide Name Circulin-A (CIRA; Plant defensin)

Sequence GIPCGESCVWIPCISAALGCSCKNKVCYRN

Sequence Length 30

UniProt ID P56871

Taxon ID None

Source Chassalia parviflora

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV-1:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=40-260 nM).

Hemolytic Activity

[Ref.10430870] EC50 = 1020 μM against blood type A human erythrocytes.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=500 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 1BH4

Predicted Structure Download DRAVPe00278

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (C termini to N termini)

Other Modification Disulfide bonds between Cys4 and Cys20, Cys8 and Cys22, Cys13 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C134H216N38O39S6

Absent amino acids DFHMQT

Common amino acids C

Mass 3175.78

Pl 8.33

Basic residues 3

Acidic residues 1

Hydrophobic residues 9

Net charge 2

Boman Index -1224

Hydrophobicity 41.67

Aliphatic Index 78

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 7365

Absorbance 280nm 253.97

Polar residues 15

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides.

Pubmed ID 10430870

Reference Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8913-8.

Author Tam JP, Lu YA, Yang JL, Chiu KW.

DOI 10.1073/pnas.96.16.8913