General Information

General Information

DRAVP ID DRAVPe00289

Peptide Name Cycloviolacin-O14 (Plant defensin)

Sequence GSIPACGESCFKGKCYTPGCSCSKYPLCAKN

Sequence Length 31

UniProt ID P85177

Source Viola odorata (Sweet violet)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=440 nM).

Hemolytic Activity

[Ref:16872274] It has 3% hemolytic activity at 1.0 μM and 13% hemolytic activity at 1.5 μM against human type A red blood cells

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=4800 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 2GJ0

Predicted Structure Download DRAVPe00289

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys6 and Cys20; Cys10 and Cys22; Cys15 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C135H212N36O42S6

Absent amino acids DHMQRVW

Common amino acids C

Mass 3203.74

Pl 8.64

Basic residues 4

Acidic residues 1

Hydrophobic residues 5

Net charge 3

Boman Index -2422

Hydrophobicity -18.71

Aliphatic Index 31.61

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 3355

Absorbance 280nm 111.83

Polar residues 18

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability.

Pubmed ID 16872274

Reference Biochem J. 2006 Nov 15;400(1):1-12.

Author Ireland DC, Colgrave ML, Craik DJ.

DOI 10.1042/BJ20060627