General Information

General Information

DRAVP ID DRAVPe00289

Peptide Name Cycloviolacin-O14 (Plant defensin)

Sequence GSIPACGESCFKGKCYTPGCSCSKYPLCAKN

Sequence Length 31

UniProt ID P85177

Taxon ID None

Source Viola odorata (Sweet violet)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=440 nM).

Hemolytic Activity

[Ref:16872274] It has 3% hemolytic activity at 1.0 μM and 13% hemolytic activity at 1.5 μM against human type A red blood cells

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=4800 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 2GJ0

Predicted Structure Download DRAVPe00289

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys6 and Cys20; Cys10 and Cys22; Cys15 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C135H212N36O42S6

Absent amino acids DHMQRVW

Common amino acids C

Mass 3203.74

Pl 8.64

Basic residues 4

Acidic residues 1

Hydrophobic residues 5

Net charge 3

Boman Index -2422

Hydrophobicity -18.71

Aliphatic Index 31.61

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 3355

Absorbance 280nm 111.83

Polar residues 18

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability.

Pubmed ID 16872274

Reference Biochem J. 2006 Nov 15;400(1):1-12.

Author Ireland DC, Colgrave ML, Craik DJ.

DOI 10.1042/BJ20060627