General Information

General Information

DRAVP ID DRAVPe00290

Peptide Name Cycloviolacin-O24 (Plant defensin)

Sequence GLPTCGETCFGGTCNTPGCTCDPWPVCTHN

Sequence Length 30

UniProt ID P84637

Taxon ID None

Source Viola odorata (Sweet violet)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=308 nM).

Hemolytic Activity

[Ref:16872274] It has 75% hemolytic activity at 25.0 μM against human type A red blood cells.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=6170 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00290

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C126H187N35O43S6

Absent amino acids AIKMQRSY

Common amino acids CT

Mass 3072.44

Pl 4.35

Basic residues 1

Acidic residues 2

Hydrophobic residues 4

Net charge -1

Boman Index -2224

Hydrophobicity -16.33

Aliphatic Index 22.67

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5875

Absorbance 280nm 202.59

Polar residues 19

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability.

Pubmed ID 16872274

Reference Biochem J. 2006 Nov 15;400(1):1-12.

Author Ireland DC, Colgrave ML, Craik DJ.

DOI 10.1042/BJ20060627