General Information

General Information

DRAVP ID DRAVPe00290

Peptide Name Cycloviolacin-O24 (Plant defensin)

Sequence GLPTCGETCFGGTCNTPGCTCDPWPVCTHN

Sequence Length 30

UniProt ID P84637

Source Viola odorata (Sweet violet)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=308 nM).

Hemolytic Activity

[Ref:16872274] It has 75% hemolytic activity at 25.0 μM against human type A red blood cells.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=6170 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00290

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C126H187N35O43S6

Absent amino acids AIKMQRSY

Common amino acids CT

Mass 3072.44

Pl 4.35

Basic residues 1

Acidic residues 2

Hydrophobic residues 4

Net charge -1

Boman Index -2224

Hydrophobicity -16.33

Aliphatic Index 22.67

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5875

Absorbance 280nm 202.59

Polar residues 19

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability.

Pubmed ID 16872274

Reference Biochem J. 2006 Nov 15;400(1):1-12.

Author Ireland DC, Colgrave ML, Craik DJ.

DOI 10.1042/BJ20060627