General Information

General Information

DRAVP ID DRAVPe00291

Peptide Name Cycloviolacin-Y1 (Plants)

Sequence GGTIFDCGETCFLGTCYTPGCSCGNYGFCYGTN

Sequence Length 33

UniProt ID No entry found

Source Viola yedoensis (Chinese herb)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18081258] Anti-HIV activity:EC50=1.2μM, IC50>4.5μM.
NOTE:EC50 refers to the values for HIV-infected cell cultures, IC50 refers to the values for uninfected cell cultures.
[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=1210 nM).

Hemolytic Activity

[Ref.18081258] It has hemolytic activity.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50>4470 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00291

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys7 and Cys21; Cys11 and Cys23; Cys16 and Cys29.

Stereochemistry L

Physicochemical Information

Formula C145H203N35O49S6

Absent amino acids AHKMQRVW

Common amino acids G

Mass 3412.77

Pl 3.67

Basic residues 0

Acidic residues 2

Hydrophobic residues 5

Net charge -2

Boman Index -1150

Hydrophobicity 14.24

Aliphatic Index 23.64

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 4845

Absorbance 280nm 151.41

Polar residues 25

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis.

Pubmed ID 18081258

Reference J Nat Prod. 2008 Jan;71(1):47-52.

Author Wang CK, Colgrave ML, Gustafson KR, Ireland DC, Goransson U, Craik DJ.

DOI 10.1021/np070393g