General Information

General Information

DRAVP ID DRAVPe00291

Peptide Name Cycloviolacin-Y1 (Plants)

Sequence GGTIFDCGETCFLGTCYTPGCSCGNYGFCYGTN

Sequence Length 33

UniProt ID No entry found

Taxon ID None

Source Viola yedoensis (Chinese herb)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18081258] Anti-HIV activity:EC50=1.2μM, IC50>4.5μM.
NOTE:EC50 refers to the values for HIV-infected cell cultures, IC50 refers to the values for uninfected cell cultures.
[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=1210 nM).

Hemolytic Activity

[Ref.18081258] It has hemolytic activity.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50>4470 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00291

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys7 and Cys21; Cys11 and Cys23; Cys16 and Cys29.

Stereochemistry L

Physicochemical Information

Formula C145H203N35O49S6

Absent amino acids AHKMQRVW

Common amino acids G

Mass 3412.77

Pl 3.67

Basic residues 0

Acidic residues 2

Hydrophobic residues 5

Net charge -2

Boman Index -1150

Hydrophobicity 14.24

Aliphatic Index 23.64

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 4845

Absorbance 280nm 151.41

Polar residues 25

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis.

Pubmed ID 18081258

Reference J Nat Prod. 2008 Jan;71(1):47-52.

Author Wang CK, Colgrave ML, Gustafson KR, Ireland DC, Goransson U, Craik DJ.

DOI 10.1021/np070393g