General Information

General Information

DRAVP ID DRAVPe00293

Peptide Name Cycloviolacin-Y4 (Plants)

Sequence GVPCGESCVFIPCITGVIGCSCSSNVCYLN

Sequence Length 30

UniProt ID No entry found

Source Viola yedoensis (Chinese herb)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18618891]Effects: H. contortus( IC50=2.01μM, IC99=6.73μM) and T. colubriformis(IC50=2.27μM, IC99=5.26μM).
[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=120 nM).

Hemolytic Activity

[Ref:18081258] HD50=9.3 μM against human type A red blood cells.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=1720 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00293

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys4 and Cys20; Cys8 and Cys22; Cys13 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C127H202N32O41S6

Absent amino acids ADHKMQRW

Common amino acids C

Mass 3025.55

Pl 4

Basic residues 0

Acidic residues 1

Hydrophobic residues 9

Net charge -1

Boman Index 1386

Hydrophobicity 104.67

Aliphatic Index 90.67

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 1865

Absorbance 280nm 64.31

Polar residues 18

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis.

Pubmed ID 18081258

Reference J Nat Prod. 2008 Jan;71(1):47-52.

Author Wang CK, Colgrave ML, Gustafson KR, Ireland DC, Goransson U, Craik DJ.

DOI 10.1021/np070393g