General Information

General Information

DRAVP ID DRAVPe00293

Peptide Name Cycloviolacin-Y4 (Plants)

Sequence GVPCGESCVFIPCITGVIGCSCSSNVCYLN

Sequence Length 30

UniProt ID No entry found

Taxon ID None

Source Viola yedoensis (Chinese herb)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18618891]Effects: H. contortus( IC50=2.01μM, IC99=6.73μM) and T. colubriformis(IC50=2.27μM, IC99=5.26μM).
[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=120 nM).

Hemolytic Activity

[Ref:18081258] HD50=9.3 μM against human type A red blood cells.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=1720 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00293

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (N termini to C termini)

Other Modification Disulfide bonds between Cys4 and Cys20; Cys8 and Cys22; Cys13 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C127H202N32O41S6

Absent amino acids ADHKMQRW

Common amino acids C

Mass 3025.55

Pl 4

Basic residues 0

Acidic residues 1

Hydrophobic residues 9

Net charge -1

Boman Index 1386

Hydrophobicity 104.67

Aliphatic Index 90.67

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 1865

Absorbance 280nm 64.31

Polar residues 18

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis.

Pubmed ID 18081258

Reference J Nat Prod. 2008 Jan;71(1):47-52.

Author Wang CK, Colgrave ML, Gustafson KR, Ireland DC, Goransson U, Craik DJ.

DOI 10.1021/np070393g