General Information

General Information

DRAVP ID DRAVPe00297

Peptide Name Kalata-B8 (Plant defensin)

Sequence GSVLNCGETCLLGTCYTTGCTCNKYRVCTKD

Sequence Length 31

UniProt ID P85175

Source Oldenlandia affinis

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=2500 nM).

Hemolytic Activity

[Ref.20564013] It produced 7% hemolysis at the 62μM.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50>11000 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 2B38

Predicted Structure Download DRAVPe00297

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (C termini to N termini)

Other Modification Disulfide bonds between Cys6 and Cys20; Cys10 and Cys22; Cys15 and Cys28.

Stereochemistry L

Physicochemical Information

Formula C134H220N38O47S6

Absent amino acids AFHIMPQW

Common amino acids CT

Mass 3307.81

Pl 7.76

Basic residues 3

Acidic residues 2

Hydrophobic residues 5

Net charge 1

Boman Index -3965

Hydrophobicity -2.26

Aliphatic Index 56.45

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 3355

Absorbance 280nm 111.83

Polar residues 21

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Structural and biochemical characteristics of the cyclotide kalata B5 from Oldenlandia affinis.

Pubmed ID 20564013

Reference Biopolymers. 2010;94(5):647-58.

Author Plan MR, Rosengren KJ, Sando L, Daly NL, Craik DJ.

DOI 10.1002/bip.21409