General Information

General Information

DRAVP ID DRAVPe00297

Peptide Name Kalata-B8 (Plant defensin)

Sequence GSVLNCGETCLLGTCYTTGCTCNKYRVCTKD

Sequence Length 31

UniProt ID P85175

Taxon ID None

Source Oldenlandia affinis

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=2500 nM).

Hemolytic Activity

[Ref.20564013] It produced 7% hemolysis at the 62μM.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50>11000 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 2B38

Predicted Structure Download DRAVPe00297

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (C termini to N termini)

Other Modification Disulfide bonds between Cys6 and Cys20; Cys10 and Cys22; Cys15 and Cys28.

Stereochemistry L

Physicochemical Information

Formula C134H220N38O47S6

Absent amino acids AFHIMPQW

Common amino acids CT

Mass 3307.81

Pl 7.76

Basic residues 3

Acidic residues 2

Hydrophobic residues 5

Net charge 1

Boman Index -3965

Hydrophobicity -2.26

Aliphatic Index 56.45

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 3355

Absorbance 280nm 111.83

Polar residues 21

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Structural and biochemical characteristics of the cyclotide kalata B5 from Oldenlandia affinis.

Pubmed ID 20564013

Reference Biopolymers. 2010;94(5):647-58.

Author Plan MR, Rosengren KJ, Sando L, Daly NL, Craik DJ.

DOI 10.1002/bip.21409