General Information

General Information

DRAVP ID DRAVPe00298

Peptide Name Varv peptide E (Varv E; Plant defensin)

Sequence GLPICGETCVGGTCNTPGCSCSWPVCTRN

Sequence Length 29

UniProt ID P83835

Taxon ID None

Source Viola arvensis (European field pansy) (Field violet)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=350 nM).

Hemolytic Activity

[Ref.20580652] HD50=6.96 µM against human type O red blood cells.

Cytotoxicity

[Ref.20580652] Cytotoxicity: U251(IC50=38.84μg/mL), MDA-MB-231(IC50>10μg/mL), A549(IC50>10μg/mL), DU145(IC50>10μg/mL), BEL-7402(IC50>10μg/mL).
[Ref.14987049] Cytotoxicity: U-937 GTB (lymphoma)(IC50 =

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00298

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (C termini to N termini)

Other Modification Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys26.

Stereochemistry L

Physicochemical Information

Formula C117H187N35O40S6

Absent amino acids ADFHKMQY

Common amino acids C

Mass 2916.34

Pl 5.96

Basic residues 1

Acidic residues 1

Hydrophobic residues 5

Net charge 0

Boman Index -1946

Hydrophobicity 15.86

Aliphatic Index 46.9

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5875

Absorbance 280nm 209.82

Polar residues 19

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Isolation and characterization of cytotoxic cyclotides from Viola tricolor.

Pubmed ID 20580652

Reference Peptides. 2010 Aug;31(8):1434-40.

Author Tang J, Wang CK, Pan X, Yan H, Zeng G, Xu W, He W, Daly NL, Craik DJ, Tan N.

DOI 10.1016/j.peptides.2010.05.004