General Information

General Information

DRAVP ID DRAVPe00298

Peptide Name Varv peptide E (Varv E; Plant defensin)

Sequence GLPICGETCVGGTCNTPGCSCSWPVCTRN

Sequence Length 29

UniProt ID P83835

Source Viola arvensis (European field pansy) (Field violet)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=350 nM).

Hemolytic Activity

[Ref.20580652] HD50=6.96 µM against human type O red blood cells.

Cytotoxicity

[Ref.20580652] Cytotoxicity: U251(IC50=38.84μg/mL), MDA-MB-231(IC50>10μg/mL), A549(IC50>10μg/mL), DU145(IC50>10μg/mL), BEL-7402(IC50>10μg/mL).
[Ref.14987049] Cytotoxicity: U-937 GTB (lymphoma)(IC50 =

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00298

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (C termini to N termini)

Other Modification Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys26.

Stereochemistry L

Physicochemical Information

Formula C117H187N35O40S6

Absent amino acids ADFHKMQY

Common amino acids C

Mass 2916.34

Pl 5.96

Basic residues 1

Acidic residues 1

Hydrophobic residues 5

Net charge 0

Boman Index -1946

Hydrophobicity 15.86

Aliphatic Index 46.9

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5875

Absorbance 280nm 209.82

Polar residues 19

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Isolation and characterization of cytotoxic cyclotides from Viola tricolor.

Pubmed ID 20580652

Reference Peptides. 2010 Aug;31(8):1434-40.

Author Tang J, Wang CK, Pan X, Yan H, Zeng G, Xu W, He W, Daly NL, Craik DJ, Tan N.

DOI 10.1016/j.peptides.2010.05.004