General Information

General Information

DRAVP ID DRAVPe00303

Peptide Name Human defensin-6 (HD-6; Defensin, alpha 6; Human, mammals, animals)

Sequence AFTCHCRRSCYSTEYSYGTCTVMGINHRFCCL

Sequence Length 32

UniProt ID Q01524

Source Homo sapiens (Human)

Activity Information

Target Organism SARS-CoV-2

Assay

Activity

[Ref.34206990]SARS-CoV-2:inhibition of infection in HEK293T-hACE2 cells(HD6 only blocked SARS-CoV-2 infection at the highest concentration tested (50 μg/mL, 13 μM)).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.34206990]No cytotoxicity on HEK293T cells up to 50 μg/mL.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 1ZMQ 3QTE

Predicted Structure Download DRAVPe00303

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Free

Other Modification Disulfide bonds between Cys4 and Cys31,Cys6 and Cys20,Cys10 and Cys30.

Stereochemistry L

Physicochemical Information

Formula C156H234N46O46S7

Absent amino acids DKPQW

Common amino acids C

Mass 3714.27

Pl 8.35

Basic residues 5

Acidic residues 1

Hydrophobic residues 6

Net charge 4

Boman Index -5487

Hydrophobicity 0

Aliphatic Index 36.56

Half Life

Mammalian:4.4 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 4845

Absorbance 280nm 156.29

Polar residues 19

Literature Information

Literature 1

Title Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry.

Pubmed ID 34206990

Reference Viruses. 2021 Jun 26;13(7):1246.

Author Xu C, Wang A, Marin M, Honnen W, Ramasamy S, Porter E, Subbian S, Pinter A, Melikyan GB, Lu W, Chang TL.

DOI 10.3390/v13071246