General Information

General Information

DRAVP ID DRAVPe00341

Peptide Name M-zodatoxin-Lt3a (M-ZDTX-Lt3a; Latarcin-3a, Ltc-3a; spiders, Arthropods, animals)

Sequence SWKSMAKKLKEYMEKLKQRA

Sequence Length 20

UniProt ID Q1ELU3

Taxon ID None

Source Lachesana tarabaevi (Spider)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV

Assay

Activity

[Ref.20086159]HIV-1 IIIB:inhibition the cytopathic effects of HIV-1 infection in CEM-SS cells(EC50=32.7 μM).

Hemolytic Activity

[Ref.16735513]20% hemolytic activity at >120 μM against rabbit erythrocytes

Cytotoxicity

[Ref.20086159]CEM-SS cells:TC50(cause 50% Cell death) >40.3 µM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00341

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C111H187N31O29S2

Absent amino acids CDFGHINPTV

Common amino acids K

Mass 2484.02

Pl 10.12

Basic residues 7

Acidic residues 2

Hydrophobic residues 5

Net charge 5

Boman Index -5383

Hydrophobicity -136

Aliphatic Index 49

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 6990

Absorbance 280nm 367.89

Polar residues 3

Literature Information

Literature 1

Title Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database.

Pubmed ID 20086159

Reference Antimicrob Agents Chemother. 2010 Mar;54(3):1343-6.

Author Wang G, Watson KM, Peterkofsky A, Buckheit RW Jr.

DOI 10.1128/AAC.01448-09

Literature 2

Title Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity.

Pubmed ID 16735513

Reference J Biol Chem. 2006 Jul 28;281(30):20983-209892.

Author Kozlov SA, Vassilevski AA, Feofanov AV, Surovoy AY, Karpunin DV, Grishin EV.

DOI 10.1074/jbc.M602168200