General Information

General Information

DRAVP ID DRAVPe00374

Peptide Name Ranatuerin-2P (Ranatuerin 2P; Frogs, amphibians, animals)

Sequence GLMDTVKNVAKNLAGHMLDKLKCKITGC

Sequence Length 28

UniProt ID Q8QFQ4 P82847

Taxon ID None

Source Rana pipiens (Northern leopard frog)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism FV3,CCV

Assay

Activity

[Ref.11601906]Frog Virus 3(FV3): inhibition of FV3 infection in fathead minnow(FHM) cells(90% inhibition at 500 µM);
Channel Catfish virus(CCV): inhibition of CCV infection in catfish ovary(CCO) cells(99% inhibition at 50 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 2K10

Predicted Structure Download DRAVPe00374

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Cyclization of a C-terminal Cys residue (forming a disulfide bond)

Other Modification There is a disulfide bond between Cys22 and Cys27.

Stereochemistry L

Physicochemical Information

Formula C128H225N37O37S4

Absent amino acids EFPQRSWY

Common amino acids K

Mass 3002.66

Pl 9.24

Basic residues 6

Acidic residues 2

Hydrophobic residues 9

Net charge 4

Boman Index -2189

Hydrophobicity 4.29

Aliphatic Index 97.5

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 125

Absorbance 280nm 4.63

Polar residues 9

Literature Information

Literature 1

Title Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin.

Pubmed ID 11601906

Reference Virology. 2001 Sep 30;288(2):351-7.

Author Chinchar VG, Wang J, Murti G, Carey C, Rollins-Smith L.

DOI 10.1006/viro.2001.1080