General Information

General Information

DRAVP ID DRAVPe00375

Peptide Name Esculentin-2P (Frogs, amphibians, animals)

Sequence GFLSIFRGVAKFASKGLGKDLARLGVNLVACKISKQC

Sequence Length 37

UniProt ID P82846

Source Rana pipiens (northern leopard frog)

Activity Information

Target Organism FV3,CCV

Assay

Activity

[Ref.11601906]
Frog Virus 3: inhibition of FV3 infection in fathead minnow(FHM) cells(90% inhibition at 500 µM);
Channel Catfish virus(CCV): inhibition of CCV infection in catfish ovary(CCO) cells( 90% inhibition at 50 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00375

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Cyclization of a C-terminal Cys residue (forming a disulfide bond)

Other Modification There is a disulfide bond between Cys31 and Cys37.

Stereochemistry L

Physicochemical Information

Formula C176H296N50O45S2

Absent amino acids EHMPTWY

Common amino acids GKL

Mass 3896.71

Pl 10.21

Basic residues 7

Acidic residues 1

Hydrophobic residues 17

Net charge 6

Boman Index -1869

Hydrophobicity 48.11

Aliphatic Index 108.11

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 125

Absorbance 280nm 3.47

Polar residues 11

Literature Information

Literature 1

Title Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin.

Pubmed ID 11601906

Reference Virology. 2001 Sep 30;288(2):351-7.

Author Chinchar VG, Wang J, Murti G, Carey C, Rollins-Smith L.

DOI 10.1006/viro.2001.1080