General Information


DRAVP ID  DRAVPe00375

Peptide Name   Esculentin-2P (Frogs, amphibians, animals)

Sequence  GFLSIFRGVAKFASKGLGKDLARLGVNLVACKISKQC

Sequence Length  37

UniProt ID  P82846 

Source  Rana pipiens (northern leopard frog)



Activity Information


Target Organism  FV3,CCV

Assay 

Activity 

  • [Ref.11601906]
  • Frog Virus 3: inhibition of FV3 infection in fathead minnow(FHM) cells(90% inhibition at 500 µM);
  • Channel Catfish virus(CCV): inhibition of CCV infection in catfish ovary(CCO) cells( 90% inhibition at 50 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  Not found

Mechanism  No machanism information found in the reference(s) presented in this entry



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00375

Linear/Cyclic  Cyclic

N-terminal Modification  Free

C-terminal Modification  Cyclization of a C-terminal Cys residue (forming a disulfide bond)

Other Modification  There is a disulfide bond between Cys31 and Cys37.

Stereochemistry  L



Physicochemical Information


Formula  C176H296N50O45S2

Absent amino acids  EHMPTWY

Common amino acids  GKL

Mass  3896.71

Pl  10.21

Basic residues  7

Acidic residues  1

Hydrophobic residues  17

Net charge  6

Boman Index  -1869

Hydrophobicity  48.11

Aliphatic Index  108.11

Half Life 

  •     Mammalian:30 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  125

Absorbance 280nm  3.47

Polar residues  11



Literature Information


Literature 1

Title   Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin.

Pubmed ID   11601906

Reference   Virology. 2001 Sep 30;288(2):351-7.

Author   Chinchar VG, Wang J, Murti G, Carey C, Rollins-Smith L.

DOI   10.1006/viro.2001.1080