General Information


DRAVP ID  DRAVPe00376

Peptide Name   Temporin L(TL)

Sequence  FVQWFSKFLGRIL

Sequence Length  13

UniProt ID  P57104 

Source  Rana temporaria (European common frog)



Activity Information


Target Organism  HSV

Assay 

Activity 

  • [Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=8.55 μM,IC90=15.66 μM);
  • HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=8.28 μM μM,IC90=16.04 μM).

Hemolytic Activity 

  • [Ref.35216177]showing residual hemolytic activity against human erythrocytes only at concentrations equal or above 50 μM.

Cytotoxicity 

  • [Ref.35216177]Vero cells:CC50= 19.61 μM

Binding Target  membrane

Mechanism  TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.



Structure Information


PDB ID  6GS5 

Predicted Structure Download  DRAVPe00376

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C83H121N19O16

Absent amino acids  ACDEHMNPTY

Common amino acids  F

Mass  1640.99

Pl  11

Basic residues  2

Acidic residues  0

Hydrophobic residues  8

Net charge  2

Boman Index  160

Hydrophobicity  82.31

Aliphatic Index  112.31

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  5500

Absorbance 280nm  458.33

Polar residues  2



Literature Information


Literature 1

Title   Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID   35216177

Reference   Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author   Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M. 

DOI   10.3390/ijms23042060