DRAVP ID  DRAVPe00376

Peptide Name   Temporin L(TL)

Sequence  FVQWFSKFLGRIL

Sequence Length  13

UniProt ID  P57104 

Taxon ID  None

Source  Rana temporaria (European common frog)

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not available

Target Organism  HSV

Assay 

Activity 

• [Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=8.55 μM,IC90=15.66 μM);
• HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=8.28 μM μM,IC90=16.04 μM).

Hemolytic Activity 

• [Ref.35216177]showing residual hemolytic activity against human erythrocytes only at concentrations equal or above 50 μM.

Cytotoxicity 

• [Ref.35216177]Vero cells:CC50= 19.61 μM

Binding Target  membrane

Mechanism  TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

PDB ID  6GS5 

Predicted Structure Download  DRAVPe00376

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L

Literature 1

Title   Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID   35216177

Reference   Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author   Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M. 

DOI   10.3390/ijms23042060