General Information

General Information

DRAVP ID DRAVPe00376

Peptide Name Temporin L(TL)

Sequence FVQWFSKFLGRIL

Sequence Length 13

UniProt ID P57104

Source Rana temporaria (European common frog)

Activity Information

Target Organism HSV

Assay

Activity

[Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=8.55 μM,IC90=15.66 μM);
HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=8.28 μM μM,IC90=16.04 μM).

Hemolytic Activity

[Ref.35216177]showing residual hemolytic activity against human erythrocytes only at concentrations equal or above 50 μM.

Cytotoxicity

[Ref.35216177]Vero cells:CC50= 19.61 μM

Binding Target membrane

Mechanism TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

Structure Information

PDB ID 6GS5

Predicted Structure Download DRAVPe00376

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C83H121N19O16

Absent amino acids ACDEHMNPTY

Common amino acids F

Mass 1640.99

Pl 11

Basic residues 2

Acidic residues 0

Hydrophobic residues 8

Net charge 2

Boman Index 160

Hydrophobicity 82.31

Aliphatic Index 112.31

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 5500

Absorbance 280nm 458.33

Polar residues 2

Literature Information

Literature 1

Title Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID 35216177

Reference Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M.

DOI 10.3390/ijms23042060