General Information

General Information

DRAVP ID DRAVPe00377

Peptide Name Temporin L[Pro3,DLeu9](TL1)

Sequence FVPWFSKFlGRIL

Sequence Length 13

UniProt ID No entry found

Taxon ID None

Source Synthetic construct(derived from Temporin-L)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism SARS-CoV-2,HSV,HCoV-OC43,HPIV-3,HCoV-229E

Assay

Activity

[Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=9.99 μM,IC90=18.69 μM);
HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=8.86 μM μM,IC90=16.71 μM);
SARS-CoV-2:inhibition of replication in Vero cells(IC50=4.62 μM μM,IC90=12.14 μM);
HPIV-3:inhibition of replication in Vero cells(IC50>50.00 μM,IC90>50.00 μM);
HCoV-229E:inhibition of replication in Vero cells(IC50=7.76 μM,IC90=30.07 μM);
HCoV-OC43:inhibition of replication in Vero cells(IC50=7.33 μM,IC90=14.98 μM).

Hemolytic Activity

[Ref.35216177]showing residual hemolytic activity against human erythrocytes only at concentrations equal or above 50 μM.

Cytotoxicity

[Ref.35216177]Vero cells:CC50= 42.18 μM

Binding Target membrane

Mechanism TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry Mixed(D-Leu9)

Physicochemical Information

Formula C77H107N17O13

Absent amino acids ACDEHMNQTY

Common amino acids F

Mass 1609.98

Pl 11

Basic residues 2

Acidic residues 0

Hydrophobic residues 7

Net charge 2

Boman Index 222

Hydrophobicity 67.69

Aliphatic Index 82.31

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 5500

Absorbance 280nm 458.33

Polar residues 2

Literature Information

Literature 1

Title Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID 35216177

Reference Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M.

DOI 10.3390/ijms23042060