General Information


DRAVP ID  DRAVPe00384

Peptide Name   Temporin L[Pro3,DLeu9,DLys10](TL9)

Sequence  FVPWFSKFlkRIL

Sequence Length  13

UniProt ID  No entry found

Source  Synthetic construct(derived from Temporin-L)



Activity Information


Target Organism  SARS-CoV-2,HSV,HCoV-OC43,HPIV-3,HCoV-229E

Assay 

Activity 

  • [Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=3.53 μM,IC90=12.71 μM);
  • HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=2.80 μM μM,IC90=12.50 μM);
  • SARS-CoV-2:inhibition of replication in Vero cells(IC50=4.63 μM μM,IC90=25.33 μM);
  • HPIV-3:inhibition of replication in Vero cells(IC50=40.64 μM,IC90>50.00 μM);
  • HCoV-229E:inhibition of replication in Vero cells(IC50=5.39 μM,IC90=50.00 μM);
  • HCoV-OC43:inhibition of replication in Vero cells(IC50=8.29 μM,IC90=29.17 μM).

Hemolytic Activity 

  • [Ref.35216177]<20% hemolysis against human erythrocytes at concentrations equal or above 25 μM.

Cytotoxicity 

  • [Ref.35216177]Vero cells:CC50= 45.92 μM

Binding Target  membrane

Mechanism  TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  Mixed(D-Leu9,D-Lys10)



Physicochemical Information


Formula  C75H102N16O11

Absent amino acids  ACDEGHMNQTY

Common amino acids  F

Mass  1681.1

Pl  11.17

Basic residues  2

Acidic residues  0

Hydrophobic residues  7

Net charge  2

Boman Index  128

Hydrophobicity  70.77

Aliphatic Index  82.31

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  5500

Absorbance 280nm  458.33

Polar residues  1



Literature Information


Literature 1

Title   Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID   35216177

Reference   Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author   Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M. 

DOI   10.3390/ijms23042060