General Information

General Information

DRAVP ID DRAVPe00387

Peptide Name Temporin L[Pro3,DLeu9,Aic10](TL12)

Sequence FVPWFSKFlXRIL

Sequence Length 13

UniProt ID No entry found

Source Synthetic construct(derived from Temporin-L)

Activity Information

Target Organism SARS-CoV-2,HSV,HCoV-OC43,HPIV-3,HCoV-229E

Assay

Activity

[Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=0.68 μM,IC90=1.58 μM);
HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=0.54 μM μM,IC90=11.86 μM);
SARS-CoV-2:inhibition of replication in Vero cells(IC50=0.65 μM μM,IC90=13.85 μM);
HPIV-3:inhibition of replication in Vero cells(IC50=33.83 μM,IC90>50.00 μM);
HCoV-229E:inhibition of replication in Vero cells(IC50=0.74 μM,IC90=20.90 μM);
HCoV-OC43:inhibition of replication in Vero cells(IC50=0.62 μM,IC90=13.18 μM).

Hemolytic Activity

[Ref.35216177]<20% hemolysis against human erythrocytes at concentrations equal or above 12.5 μM.

Cytotoxicity

[Ref.35216177]Vero cells:CC50=8.28 μM

Binding Target membrane

Mechanism TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification The 'X' at position 10 is 2-aminoindane-2-carboxylic acid.

Stereochemistry Mixed(D-Leu9)

Physicochemical Information

Formula C75H102N16O11

Absent amino acids ACDEGHMNQTY

Common amino acids F

Mass 1664.26

Pl 11

Basic residues 2

Acidic residues 0

Hydrophobic residues 7

Net charge 2

Boman Index 128

Hydrophobicity 70.77

Aliphatic Index 82.31

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 5500

Absorbance 280nm 458.33

Polar residues 1

Literature Information

Literature 1

Title Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID 35216177

Reference Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M.

DOI 10.3390/ijms23042060