General Information

General Information

DRAVP ID DRAVPe00388

Peptide Name Temporin L[Pro3,DLeu9,Nle10](TL6)

Sequence FVPWFSKFlXRIL

Sequence Length 13

UniProt ID No entry found

Taxon ID None

Source Synthetic construct(derived from Temporin-L)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism SARS-CoV-2,HSV,HCoV-OC43,HPIV-3,HCoV-229E,MeV,H1N1

Assay

Activity

[Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=2.66 μM,IC90=9.12 μM);
HSV-2:inhibition of HSV-2 replication in Vero cells(IC50=0.92 μM μM,IC90=6.28 μM);
SARS-CoV-2:inhibition of replication in Vero cells(IC50=0.53 μM μM,IC90=12.15 μM);
HPIV-3:inhibition of replication in Vero cells(IC50=32.35 μM,IC90>50.00 μM);
HCoV-229E:inhibition of replication in Vero cells(IC50=2.08 μM,IC90=13.06 μM);
HCoV-OC43:inhibition of replication in Vero cells(IC50=0.97 μM,IC90=10.04 μM);
Measles virus(MeV):inhibition of MeV replication in VERO/hSLAM cells(IC50=34.58 μM μM,IC90>50.00 μM);
influenza virus(H1N1,VR-1894):inhibition of replication in MDKC cells(IC50=2.66 μM,IC90=9.12 μM).

Hemolytic Activity

[Ref.35216177]<20% hemolysis against human erythrocytes at concentrations equal or above 25 μM.

Cytotoxicity

[Ref.35216177]Vero cells:CC50=64.52 μM

Binding Target membrane

Mechanism TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification The 'X' at position 10 is norleucine.

Stereochemistry Mixed(D-Leu9)

Physicochemical Information

Formula C75H102N16O11

Absent amino acids ACDEGHMNQTY

Common amino acids F

Mass 1664.26

Pl 11

Basic residues 2

Acidic residues 0

Hydrophobic residues 7

Net charge 2

Boman Index 128

Hydrophobicity 70.77

Aliphatic Index 82.31

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 5500

Absorbance 280nm 458.33

Polar residues 1

Literature Information

Literature 1

Title Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID 35216177

Reference Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M.

DOI 10.3390/ijms23042060