DRAVP ID  DRAVPe00390

Peptide Name   Temporin L[Pro3,DLeu9,Nle10]-GGC-CHOL(TL6.2)

Sequence  FVPWFSKFlXRILGGC

Sequence Length  16

UniProt ID  No entry found

Source  Synthetic construct(derived from Temporin-L)

Target Organism  SARS-CoV-2,HSV, MeV,H1N1

Assay 

Activity 

• [Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=9.54 μM,IC90=47.95 μM);
• SARS-CoV-2:inhibition of replication in Vero cells(IC50=12.12 μM μM,IC90=49.95 μM);
• Measles virus(MeV):inhibition of MeV replication in VERO/hSLAM cells(IC50=40.89 μM μM,IC90>50.00 μM);
• influenza virus(H1N1,VR-1894):inhibition of replication in MDKC cells(IC50=21.12 μM,IC90=49.90 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.35216177]Vero cells:CC50>100.00 μM

Binding Target  membrane

Mechanism  TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  PEG4-Cholesterol-NH2

Other Modification  The 'X' at position 10 is norleucine.

Stereochemistry  Mixed(D-Leu9)

Literature 1

Title   Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID   35216177

Reference   Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author   Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M. 

DOI   10.3390/ijms23042060