General Information

General Information

DRAVP ID DRAVPe00391

Peptide Name CHOL-C-Temporin L[Pro3,DLeu9,Nle10](TL6.3)

Sequence CFVPWFSKFlXRIL

Sequence Length 14

UniProt ID No entry found

Source Synthetic construct(derived from Temporin-L)

Activity Information

Target Organism SARS-CoV-2,HSV, MeV,H1N1

Assay

Activity

[Ref.35216177]HSV-1:inhibition of HSV-1 replication in Vero cells(IC50=0.89 μM,IC90=2.19 μM);
SARS-CoV-2:inhibition of replication in Vero cells(IC50=0.76 μM μM,IC90=1.89 μM);
Measles virus(MeV):inhibition of MeV replication in VERO/hSLAM cells(IC50=22.3 μM μM,IC90=31.10 μM);
influenza virus(H1N1,VR-1894):inhibition of replication in MDKC cells(IC50=2.55 μM,IC90=5.89 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.35216177]Vero cells:CC50>100.00 μM

Binding Target membrane

Mechanism TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Cholesterol-PEG4

C-terminal Modification Amidation

Other Modification The 'X' at position 10 is norleucine.

Stereochemistry Mixed(D-Leu9)

Physicochemical Information

Formula C78H107N17O12S

Absent amino acids ADEGHMNQTY

Common amino acids F

Mass 1767.39

Pl 9.51

Basic residues 2

Acidic residues 0

Hydrophobic residues 7

Net charge 2

Boman Index 256

Hydrophobicity 83.57

Aliphatic Index 76.43

Half Life

Mammalian:1.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5500

Absorbance 280nm 423.08

Polar residues 2

Literature Information

Literature 1

Title Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

Pubmed ID 35216177

Reference Int J Mol Sci. 2022 Feb 13;23(4):2060.

Author Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M.

DOI 10.3390/ijms23042060