General Information


DRAVP ID  DRAVPe00423

Peptide Name   IBP21

Sequence  SVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIK

Sequence Length  37

UniProt ID  No entry found

Source  Synthetic construct(derived from HR2 region of SARS-CoV-2)



Activity Information


Target Organism  SARS-CoV-2

Assay 

Activity 

  • [Ref.34057039]SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=7.5 nM);inhibition of pseudovirus infections in 293T/ACE2 cells(IC50=191.4 nM);inhibition of pseudovirus infections in Huh-7 cells(IC50=126.65 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  membrane

Mechanism  Peptides derived from HR2 sequences of viral fusion proteins, including the S protein of emerging CoVs, can competitively bind to the HR1 domain and block the formation of the viral 6-HB core, thereby inhibiting infection of the virus from which they were derived.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  C16(palmitic acid)

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C192H317N51O63

Absent amino acids  CFHMPTW

Common amino acids  EL

Mass  4347.93

Pl  4.77

Basic residues  5

Acidic residues  7

Hydrophobic residues  13

Net charge  -2

Boman Index  -7972

Hydrophobicity  -60.27

Aliphatic Index  121.08

Half Life 

  •     Mammalian:1.9 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  2980

Absorbance 280nm  82.78

Polar residues  9



Literature Information


Literature 1

Title   Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variants.

Pubmed ID   34057039

Reference   Emerg Microbes Infect. 2021 Dec;10(1):1227-1240.

Author   Yu D, Zhu Y, Jiao T, Wu T, Xiao X, Qin B, Chong H, Lei X, Ren L, Cui S, Wang J, He Y.

DOI   10.1080/22221751.2021.1937329