DRAVP ID  DRAVPe00426

Peptide Name   IBP24

Sequence  SVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIK

Sequence Length  37

UniProt ID  No entry found

Source  Synthetic construct(derived from HR2 region of SARS-CoV-2)

Target Organism  SARS-CoV-2,SARS-CoV,MERS-CoV,HCoV-NL63

Assay 

Activity 

• [Ref.34057039]SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=0.33 nM);inhibition of pseudovirus infections in 293T/ACE2 cells(IC50=3.77 nM);inhibition of pseudovirus infections in Huh-7 cells(IC50=2.89 nM);inhibition of live SARS-CoV-2 infection in Vero cells(IC50=8.97 nM);
• SARS-CoV-2 D614G:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.29 nM);
• SARS-CoV-2 N501Y:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.5 nM);
• SARS-CoV-2 ΔH69-V70:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.42 nM);
• SARS-CoV-2 E484K:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.97 nM);
• SARS-CoV-2 B.1.1.7:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.22 nM);
• SARS-CoV-2 B.1.351:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.06 nM);
• SARS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=21.64 nM);
• MERS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=69.9 nM);
• HCoV-NL63:inhibition of pseudovirus infections in Huh-7 cells(IC50=375.56 nM);
• HCoV-229E:inhibition of pseudovirus infections in Huh-7 cells(IC50=421.48 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.34057039]Huh-7 cells:CC50=6.6 μM;Vero-E6 cells:CC50=13.67 μM.

Binding Target  membrane

Mechanism  Peptides derived from HR2 sequences of viral fusion proteins, including the S protein of emerging CoVs, can competitively bind to the HR1 domain and block the formation of the viral 6-HB core, thereby inhibiting infection of the virus from which they were derived.

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  PEG4-K(Chol)

Other Modification  None

Stereochemistry  L

Literature 1

Title   Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variants.

Pubmed ID   34057039

Reference   Emerg Microbes Infect. 2021 Dec;10(1):1227-1240.

Author   Yu D, Zhu Y, Jiao T, Wu T, Xiao X, Qin B, Chong H, Lei X, Ren L, Cui S, Wang J, He Y.

DOI   10.1080/22221751.2021.1937329