General Information

General Information

DRAVP ID DRAVPe00427

Peptide Name IBP25

Sequence SVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIK

Sequence Length 37

UniProt ID No entry found

Taxon ID None

Source Synthetic construct(derived from HR2 region of SARS-CoV-2)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism SARS-CoV-2,SARS-CoV,MERS-CoV,HCoV-NL63

Assay

Activity

[Ref.34057039]SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=0.29 nM);inhibition of pseudovirus infections in 293T/ACE2 cells(IC50=2.13 nM);inhibition of pseudovirus infections in Huh-7 cells(IC50=1.43 nM),inhibition of live SARS-CoV-2 infection in Vero cells(IC50=25.71 nM);
SARS-CoV-2 D614G:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.8 nM);
SARS-CoV-2 N501Y:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.52 nM);
SARS-CoV-2 ΔH69-V70:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.56 nM);
SARS-CoV-2 E484K:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.71 nM);
SARS-CoV-2 B.1.1.7:inhibition of pseudovirus infections in Huh-7 cells(IC50=5.87 nM);
SARS-CoV-2 B.1.351:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.76 nM);
SARS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=17.69 nM);
MERS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=48.5 nM);
HCoV-NL63:inhibition of pseudovirus infections in Huh-7 cells(IC50=353.22 nM);
HCoV-229E:inhibition of pseudovirus infections in Huh-7 cells(IC50=336.14 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.34057039]Huh-7 cells:CC50=3.54 μM;Vero-E6 cells:CC50=6.95 μM.

Binding Target membrane

Mechanism Peptides derived from HR2 sequences of viral fusion proteins, including the S protein of emerging CoVs, can competitively bind to the HR1 domain and block the formation of the viral 6-HB core, thereby inhibiting infection of the virus from which they were derived.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification PEG5-K(Chol)

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C192H317N51O63

Absent amino acids CFHMPTW

Common amino acids EL

Mass 4347.93

Pl 4.77

Basic residues 5

Acidic residues 7

Hydrophobic residues 13

Net charge -2

Boman Index -7972

Hydrophobicity -60.27

Aliphatic Index 121.08

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 2980

Absorbance 280nm 82.78

Polar residues 9

Literature Information

Literature 1

Title Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variants.

Pubmed ID 34057039

Reference Emerg Microbes Infect. 2021 Dec;10(1):1227-1240.

Author Yu D, Zhu Y, Jiao T, Wu T, Xiao X, Qin B, Chong H, Lei X, Ren L, Cui S, Wang J, He Y.

DOI 10.1080/22221751.2021.1937329