General Information

General Information

DRAVP ID DRAVPe00429

Peptide Name IBP27

Sequence SVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIK

Sequence Length 37

UniProt ID No entry found

Source Synthetic construct(derived from HR2 region of SARS-CoV-2)

Activity Information

Target Organism SARS-CoV-2,SARS-CoV,MERS-CoV,HCoV-NL63

Assay

Activity

[Ref.34057039]SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=0.32 nM);inhibition of pseudovirus infections in 293T/ACE2 cells(IC50=2.77 nM);inhibition of pseudovirus infections in Huh-7 cells(IC50=1.54 nM),inhibition of live SARS-CoV-2 infection in Vero cells(IC50=29.85 nM);
SARS-CoV-2 D614G:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.75 nM);
SARS-CoV-2 N501Y:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.87 nM);
SARS-CoV-2 ΔH69-V70:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.56 nM);
SARS-CoV-2 E484K:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.55 nM);
SARS-CoV-2 B.1.1.7:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.18 nM);
SARS-CoV-2 B.1.351:inhibition of pseudovirus infections in Huh-7 cells(IC50=8.25 nM);
SARS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=24.95 nM);
MERS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=60.08 nM);
HCoV-NL63:inhibition of pseudovirus infections in Huh-7 cells(IC50=179.53 nM);
HCoV-229E:inhibition of pseudovirus infections in Huh-7 cells(IC50=231.18 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.34057039]Huh-7 cells:CC50=4.04 μM;Vero-E6 cells:CC50=5.05 μM.

Binding Target membrane

Mechanism Peptides derived from HR2 sequences of viral fusion proteins, including the S protein of emerging CoVs, can competitively bind to the HR1 domain and block the formation of the viral 6-HB core, thereby inhibiting infection of the virus from which they were derived.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification PEG8-K(Chol)

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C192H317N51O63

Absent amino acids CFHMPTW

Common amino acids EL

Mass 4347.93

Pl 4.77

Basic residues 5

Acidic residues 7

Hydrophobic residues 13

Net charge -2

Boman Index -7972

Hydrophobicity -60.27

Aliphatic Index 121.08

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 2980

Absorbance 280nm 82.78

Polar residues 9

Literature Information

Literature 1

Title Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variants.

Pubmed ID 34057039

Reference Emerg Microbes Infect. 2021 Dec;10(1):1227-1240.

Author Yu D, Zhu Y, Jiao T, Wu T, Xiao X, Qin B, Chong H, Lei X, Ren L, Cui S, Wang J, He Y.

DOI 10.1080/22221751.2021.1937329