General Information


DRAVP ID  DRAVPe00429

Peptide Name   IBP27

Sequence  SVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIK

Sequence Length  37

UniProt ID  No entry found

Source  Synthetic construct(derived from HR2 region of SARS-CoV-2)



Activity Information


Target Organism  SARS-CoV-2,SARS-CoV,MERS-CoV,HCoV-NL63

Assay 

Activity 

  • [Ref.34057039]SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=0.32 nM);inhibition of pseudovirus infections in 293T/ACE2 cells(IC50=2.77 nM);inhibition of pseudovirus infections in Huh-7 cells(IC50=1.54 nM),inhibition of live SARS-CoV-2 infection in Vero cells(IC50=29.85 nM);
  • SARS-CoV-2 D614G:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.75 nM);
  • SARS-CoV-2 N501Y:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.87 nM);
  • SARS-CoV-2 ΔH69-V70:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.56 nM);
  • SARS-CoV-2 E484K:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.55 nM);
  • SARS-CoV-2 B.1.1.7:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.18 nM);
  • SARS-CoV-2 B.1.351:inhibition of pseudovirus infections in Huh-7 cells(IC50=8.25 nM);
  • SARS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=24.95 nM);
  • MERS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=60.08 nM);
  • HCoV-NL63:inhibition of pseudovirus infections in Huh-7 cells(IC50=179.53 nM);
  • HCoV-229E:inhibition of pseudovirus infections in Huh-7 cells(IC50=231.18 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.34057039]Huh-7 cells:CC50=4.04 μM;Vero-E6 cells:CC50=5.05 μM.

Binding Target  membrane

Mechanism  Peptides derived from HR2 sequences of viral fusion proteins, including the S protein of emerging CoVs, can competitively bind to the HR1 domain and block the formation of the viral 6-HB core, thereby inhibiting infection of the virus from which they were derived.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  PEG8-K(Chol)

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C192H317N51O63

Absent amino acids  CFHMPTW

Common amino acids  EL

Mass  4347.93

Pl  4.77

Basic residues  5

Acidic residues  7

Hydrophobic residues  13

Net charge  -2

Boman Index  -7972

Hydrophobicity  -60.27

Aliphatic Index  121.08

Half Life 

  •     Mammalian:1.9 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  2980

Absorbance 280nm  82.78

Polar residues  9



Literature Information


Literature 1

Title   Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variants.

Pubmed ID   34057039

Reference   Emerg Microbes Infect. 2021 Dec;10(1):1227-1240.

Author   Yu D, Zhu Y, Jiao T, Wu T, Xiao X, Qin B, Chong H, Lei X, Ren L, Cui S, Wang J, He Y.

DOI   10.1080/22221751.2021.1937329