General Information


DRAVP ID  DRAVPe00483

Peptide Name   LCB3

Sequence  NDDELHMLMTDLVYEALHFAKDEEIKKRVFQLFELADKAYKNNDRQKLEKVVEELKELLERLLS

Sequence Length  64

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  SARS-CoV-2

Assay  neutralization assay

Activity 

  • [Ref.32907861]SARS-CoV-2:Inhibition of infection in Vero E6 cells(IC50=48.1 pM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  spike protein

Mechanism  The peptide is a high-affinity protein minibinder to the SARS-CoV-2 spike receptor binding domain (RBD) that compete with ACE2 binding.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00483

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C346H556N90O106S2

Absent amino acids  CGPW

Common amino acids  L

Mass  7736.88

Pl  4.94

Basic residues  13

Acidic residues  16

Hydrophobic residues  24

Net charge  -3

Boman Index  -15515

Hydrophobicity  -66.25

Aliphatic Index  103.59

Half Life 

  •     Mammalian:1.4 hour
  •     Yeast:3 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  2980

Absorbance 280nm  47.3

Polar residues  7



Literature Information


Literature 1

Title   De novo design of picomolar SARS-CoV-2 miniprotein inhibitors.

Pubmed ID   32907861

Reference   Science. 2020 Oct 23;370(6515):426-431.

Author   Cao L, Goreshnik I, Coventry B, Case JB, Miller L, Kozodoy L, Chen RE, Carter L, Walls AC, Park YJ, Strauch EM, Stewart L, Diamond MS, Veesler D, Baker D.

DOI   10.1126/science.abd9909