General Information


DRAVP ID  DRAVPe00495

Peptide Name   LEDGF 361–370[K364A]

Sequence  WNSLAIDNLDV

Sequence Length  11

UniProt ID  O75475 

Source  Synthetic construct



Activity Information


Target Organism  HIV

Assay  quantitative assay(IN enzymatic activity)

Activity 

  • [Ref.20171172]HIV-1:inhibition of integrase catalytic activity(68% inhibition at 100nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  Integrase

Mechanism  The peptide inhibits the catalytic activity of integrase and thus inhibits integration of viral cDNA.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00495

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C56H86N14O19

Absent amino acids  CEFGHKMPQRTY

Common amino acids  DLN

Mass  1259.38

Pl  3.56

Basic residues  0

Acidic residues  2

Hydrophobic residues  6

Net charge  -2

Boman Index  -1118

Hydrophobicity  21.82

Aliphatic Index  141.82

Half Life 

  •     Mammalian:2.8 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  5500

Absorbance 280nm  550

Polar residues  3



Literature Information


Literature 1

Title   Mechanism of action of the HIV-1 integrase inhibitory peptide LEDGF 361-370.

Pubmed ID   20171172

Reference   Biochem Biophys Res Commun. 2010 Apr 2;394(2):260-5. 

Author   Hayouka Z, Levin A, Maes M, Hadas E, Shalev DE, Volsky DJ, Loyter A, Friedler A.

DOI   10.1016/j.bbrc.2010.02.100