General Information


DRAVP ID  DRAVPe00646

Peptide Name   peptide 4ca[E511A]

Sequence  EXINXSLXFIRXSDALLHNV

Sequence Length  20

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  RSV

Assay  rHRSV-mCherry inhibition assay

Activity 

  • [Ref.28137809]Respiratory syncytial virus (RSV):inhibition of virus infection in Hep-2 cells(EC50=1.00±0.03 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  membrane

Mechanism  The peptide disrupts the formation of the postfusion six-helix bundle required for viral cell entry.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Cyclic

N-terminal Modification  Acetylation

C-terminal Modification  Free

Other Modification  The 'X' at position 2 indicates R-pentenylalanine; The 'X' at position 5,8,12 indicates S-pentenylalanine, X(2) and X(5), X(8) and X(12) are cross-linked by hydrocarbon stapling.

Stereochemistry  L



Physicochemical Information


Formula  C82H125N23O21

Absent amino acids  CGKMPQTWY

Common amino acids  X

Mass  2286.42

Pl  5.32

Basic residues  2

Acidic residues  2

Hydrophobic residues  8

Net charge  0

Boman Index  -2176

Hydrophobicity  29.5

Aliphatic Index  117

Half Life 

  •     Mammalian:1 hour
  •     Yeast:30 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  4



Literature Information


Literature 1

Title   A Short Double-Stapled Peptide Inhibits Respiratory Syncytial Virus Entry and Spreading.

Pubmed ID   28137809

Reference   Antimicrob Agents Chemother. 2017 Mar 24;61(4):e02241-16.

Author   Gaillard V, Galloux M, Garcin D, Eléouët JF, Le Goffic R, Larcher T, Rameix-Welti MA, Boukadiri A, Héritier J, Segura JM, Baechler E, Arrell M, Mottet-Osman G, Nyanguile O.

DOI   10.1128/AAC.02241-16