General Information


DRAVP ID  DRAVPe00692

Peptide Name   P-72

Sequence  VRYLEANIEELLKKAEEQQKKNEEELKKLEK

Sequence Length  31

UniProt ID  P03377 

Source  Synthetic construct



Activity Information


Target Organism  HIV

Assay  cell-fusion assay,single-cycle infection assay

Activity 

  • [Ref.30089693]HIV-1 HXB2:inhibition of cell-cell fusion in TZM-bl cells(IC50=4.1±0.9 nM);
  • HIV-1 NL4-3:inhibition of pseudovirus infection in TZM-bl cells(IC50=16.2±3.4 nM);
  • HIV-1 JRCSF:inhibition of virus infection in TZM-bl cells(IC50=15.9±1.2 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  membrane

Mechanism  The peptide acts by binding to the heptad repeat 1 (HR1) region of gp41 and preventing the interaction of the HR1 and HR2 domains, which is required for virus–cell fusion.(By similar)



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00692

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C167H283N45O55

Absent amino acids  CDFGHMPSTW

Common amino acids  E

Mass  3801.35

Pl  5.36

Basic residues  8

Acidic residues  9

Hydrophobic residues  9

Net charge  -1

Boman Index  -10238

Hydrophobicity  -152.58

Aliphatic Index  91.29

Half Life 

  •     Mammalian:100 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  1490

Absorbance 280nm  49.67

Polar residues  3



Literature Information


Literature 1

Title   Structural and Functional Characterization of Membrane Fusion Inhibitors with Extremely Potent Activity against Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus.

Pubmed ID   30089693

Reference   J Virol. 2018 Sep 26;92(20):e01088-18.

Author   Chong H, Zhu Y, Yu D, He Y.

DOI   10.1128/JVI.01088-18