General Information

General Information

DRAVP ID DRAVPe00693

Peptide Name P-73

Sequence VEELEKKIEELLKKAEEQQKKNEEELKKLEK

Sequence Length 31

UniProt ID P03377

Source Synthetic construct

Activity Information

Target Organism HIV

Assay cell-fusion assay,single-cycle infection assay

Activity

[Ref.30089693]HIV-1 HXB2:inhibition of cell-cell fusion in TZM-bl cells(IC50=2±0.2 nM);
HIV-1 NL4-3:inhibition of pseudovirus infection in TZM-bl cells(IC50=5.6±0.7 nM);
HIV-1 JRCSF:inhibition of virus infection in TZM-bl cells(IC50=8.2±1.3 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target membrane

Mechanism The peptide acts by binding to the heptad repeat 1 (HR1) region of gp41 and preventing the interaction of the HR1 and HR2 domains, which is required for virus–cell fusion.(By similar)

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00693

Linear/Cyclic Linear

N-terminal Modification Acetylation

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C167H289N43O57

Absent amino acids CDFGHMPRSTWY

Common amino acids E

Mass 3811.39

Pl 5.11

Basic residues 9

Acidic residues 11

Hydrophobic residues 8

Net charge -2

Boman Index -10721

Hydrophobicity -176.13

Aliphatic Index 88.06

Half Life

Mammalian:100 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 1

Literature Information

Literature 1

Title Structural and Functional Characterization of Membrane Fusion Inhibitors with Extremely Potent Activity against Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus.

Pubmed ID 30089693

Reference J Virol. 2018 Sep 26;92(20):e01088-18.

Author Chong H, Zhu Y, Yu D, He Y.

DOI 10.1128/JVI.01088-18