General Information


DRAVP ID  DRAVPe00792

Peptide Name   T2635

Sequence  TTWEAWDRAIAEYAARIEALIRAAQEQQEKNEAALREL

Sequence Length  38

UniProt ID  No entry found

Source  Synthetic construct

Other Link  DRAVPa1315



Activity Information


Target Organism  HIV

Assay  MAGI/cMAGI infectivity assay

Activity 

  • [Ref.17640899]HIV IIIB:inhibition of virus infection on CEM4 cells(IC50=0.007 μg/ml);
  • HIV 098:inhibition of virus infection on CEM4 cells(IC50=0.018μg/ml);
  • HIV 098-T20:inhibition of virus infection on CEM4 cells(IC50=0.025 μg/ml);
  • HIV 098-T1249:inhibition of virus infection on CEM4 cells(IC50=0.015 μg/ml);
  • HIV 098-T651:inhibition of virus infection on CEM4 cells(IC50=0.021 μg/ml).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  membrane

Mechanism  The peptide acts by binding to the heptad repeat 1 (HR1) region of gp41 and preventing the interaction of the HR1 and HR2 domains, which is required for virus–cell fusion.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00792

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C193H305N57O62

Absent amino acids  CFGHMPSV

Common amino acids  A

Mass  4415.89

Pl  4.64

Basic residues  5

Acidic residues  8

Hydrophobic residues  18

Net charge  -3

Boman Index  -9788

Hydrophobicity  -67.11

Aliphatic Index  87.89

Half Life 

  •     Mammalian:7.2 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  12490

Absorbance 280nm  337.57

Polar residues  4



Literature Information


Literature 1

Title   Design of helical, oligomeric HIV-1 fusion inhibitor peptides with potent activity against enfuvirtide-resistant virus.

Pubmed ID   17640899

Reference   Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12772-7. 

Author   Dwyer JJ, Wilson KL, Davison DK, Freel SA, Seedorff JE, Wring SA, Tvermoes NA, Matthews TJ, Greenberg ML, Delmedico MK.

DOI   10.1073/pnas.0701478104