General Information


DRAVP ID  DRAVPe00808

Peptide Name   C41 -X(C41 C teminus-PEG conjugation)

Sequence  EWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWX

Sequence Length  42

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  HIV

Assay  cell-cell fusion inhibition assay

Activity 

  • [Ref.22770564]HIV-1:inhibition of cell-cell fusion between CHO-WT cells and K652 cells(IC50=10.9± 2.2 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  envelope glycoprotein(gp41)

Mechanism  The peptide have been shown to be effective inhibitors to prevent virus–host cell membrane fusion.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  The 'X' at position 42 indicates PEG750-Cys.

Stereochemistry  L



Physicochemical Information


Formula  C223H335N59O75

Absent amino acids  CFGMPV

Common amino acids  E

Mass  5171.81

Pl  4.22

Basic residues  4

Acidic residues  10

Hydrophobic residues  13

Net charge  -6

Boman Index  -11455

Hydrophobicity  -115

Aliphatic Index  85.95

Half Life 

  •     Mammalian:1 hour
  •     Yeast:30 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  17990

Absorbance 280nm  438.78

Polar residues  10



Literature Information


Literature 1

Title   Site-specific PEGylation of HR2 peptides: effects of PEG conjugation position and chain length on HIV-1 membrane fusion inhibition and proteolytic degradation.

Pubmed ID   22770564

Reference   Bioconjug Chem. 2012 Aug 15;23(8):1648-60.

Author   Danial M, van Dulmen TH, Aleksandrowicz J, Pötgens AJ, Klok HA.

DOI   10.1021/bc3002248