General Information

General Information

DRAVP ID DRAVPe00837

Peptide Name Melittin

Sequence GIGAVLKVLTTGLPALISWIKRKRQQ

Sequence Length 26

UniProt ID P01501

Taxon ID None

Source Apis mellifera (Honeybee)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism JV,HSV, Influenza A virus, EV-71, RSV,VSV, HIV

Assay

Activity

[Ref.15081088]Junin virus(JV):inhibtion of JV infection in Vero cells(EC50=0.86 μM);
Herpes simplex virus type 1 (HSV-1):inhibition of HSV-1 infection in Vero cells(EC50=1.35 μM);
Herpes simplex virus type 2 (HSV-2):inhibition of HSV-2 infection in Vero cells(EC50=2.05 μM).
[Ref.31422545] GFP-fused influenza A (PR8):exerts antiviral activity in MDCK cells(EC50=1.15 ± 0.09 μg/mL);
Enterovirus A71 (EV-A71):exerts antiviral activity in HeLa cells(EC50=0.76 ± 0.03 μg/mL);
GFP-fused coxsakievirus (H3):exerts antiviral activity in HeLa cells(EC50=0.99 ± 0.09 μg/mL);
GFP-fused RSV:exerts antiviral activity in HEp2 cells(EC50=0.99 ± 0.09 μg/mL);
GFP-fused VSV:exerts antiviral activity in Vero cells(EC50=1.18 ± 0.09 μg/mL);
HIV-1 (NLHX):exerts antiviral activity in Vaginal epithelial cells (VK2)(EC50=2.4 μM);
HIV-2 (NLYU2):exerts antiviral activity in Vaginal epithelial cells (VK2)(EC50=3.6 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.15081088]Vero cells:CC50=8.51 μM.

Binding Target Not found

Mechanism Melittin suppresses cell fusion mediated by HSV-1 syncytial mutants probably by interfering with the activity of the Na, K ATPase, cellular enzyme involved in the membrane fusion process.

Structure Information

PDB ID 2MLT

Predicted Structure Download DRAVPe00837

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidtion

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C131H228N38O32

Absent amino acids CDEFHMNY

Common amino acids L

Mass 2847.49

Pl 12.02

Basic residues 5

Acidic residues 0

Hydrophobic residues 12

Net charge 5

Boman Index -1482

Hydrophobicity 27.31

Aliphatic Index 135

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5500

Absorbance 280nm 220

Polar residues 6

Literature Information

Literature 1

Title Antiviral activity of antimicrobial cationic peptides against Junin virus and herpes simplex virus.

Pubmed ID 15081088

Reference Int J Antimicrob Agents. 2004 Apr;23(4):382-9.

Author Albiol Matanic VC, Castilla V.

DOI 10.1016/j.ijantimicag.2003.07.022

Literature 2

Title Melittin: a venom-derived peptide with promising anti-viral properties.

Pubmed ID 31422545

Reference Eur J Clin Microbiol Infect Dis. 2020 Jan;39(1):5-17.

Author Memariani H, Memariani M, Moravvej H, Shahidi-Dadras M.

DOI 10.1007/s10096-019-03674-0