General Information


DRAVP ID  DRAVPe00977

Peptide Name   P33(derived from HCV polyprotein)

Sequence  KKKKVVAATYVLVF

Sequence Length  14

UniProt ID  P26662 

Source  Synthetic construct(derived from Hepatitis C virus(HCV) polyprotein)



Activity Information


Target Organism  HCV

Assay  NS3-6K inhibition assay(determined by Fluorescent probe)

Activity 

  • [Ref.14694985]Hepatitis C virus(HCV):inhibition of activation of NS3-6K protease(50 nM)(12% inhibition at 3 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  NS3 protease

Mechanism  HCV NS3 protease is essential for the processing of HCV polyprotein, this protease is a target of choice to control HCV replication. The peptide was demonstrated that can indeed block the activity of NS3 and thus inhibit HCV replication



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00977

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C78H132N18O17

Absent amino acids  CDEGHIMNPQRSW

Common amino acids  KV

Mass  1594.02

Pl  10.18

Basic residues  4

Acidic residues  0

Hydrophobic residues  8

Net charge  4

Boman Index  277

Hydrophobicity  67.14

Aliphatic Index  125

Half Life 

  •     Mammalian:1.3 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  1490

Absorbance 280nm  114.62

Polar residues  2



Literature Information


Literature 1

Title   Peptide inhibitors of hepatitis C virus NS3 protease. 

Pubmed ID   14694985

Reference   Antivir Chem Chemother. 2003 Sep;14(5):225-33.

Author   Portal-Núñez S, González-Navarro CJ, García-Delgado M, Vizmanos JL, Lasarte JJ, Borrás-Cuesta F.

DOI   10.1177/095632020301400501