General Information

General Information

DRAVP ID DRAVPe01076

Peptide Name C5A(derived from HCV C5A)

Sequence SWLRDIWDWICEVLSDFK

Sequence Length 18

UniProt ID P26664

Source Synthetic construct(derived from HCV non-structural protein 5A)

Activity Information

Target Organism HCV,DENV,MeV,RSV,HIV

Assay RT-QPCR assay

Activity

[Ref.18287023]Hepatitis C virus(HCV):inhibition of HCV infection in Huh-7.5.1 cells(IC50=0.79 μM);
HCV (JFH-1) genotype 2a:inhibition of infection in Huh-7.5.1 cells(IC50=0.6 μM);
HCV (H77 envelope) genotype 1a:inhibition of infection in Huh-7.5.1 cells(IC50=3.9 μM);
HCV (Con1 envelope) genotype 1b:inhibition of infection in Huh-7.5.1 cells(IC50=1.6 μM);
HCV (J6CF envelope) genotype 2a:inhibition of infection in Huh-7.5.1 cells(IC50=1.1 μM);
Dengue virus:inhibition of infection in Huh-7.5.1 cells(IC50=2.0 μM);
West Nile virus:inhibition of infection in Huh-7.5.1 cells(IC50=4.5 μM);
Measles virus:inhibition of infection in Huh-7.5.1 cells(IC50=2.7 μM);
Respiratory syncytial virus:inhibition of infection in Huh-7.5.1 cells(IC50=4.5 μM);
Human immunodeficiency virus:inhibition of infection in Huh-7.5.1 cells(IC50=1.3 μM);
IC50>18 μM against Adenovirus,Borna disease virus,Coronavirus 229E,Coxsackie virus,Hepatitis B virus,Influenza virus,Lymphocytic choriomeningitis virus,Rhinovirus,Rotavirus WISC2,Vaccinia virus,Vesicular stomatitis virus.

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target membrane

Mechanism The peptide destabilizes viral structural integrity and has viral membranolytic activity.It interacts with the viral membrane to disrupt its integrity, release viral capsids, and expose the viral genome to exonucleases for degradation.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01076

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C109H155N25O29S

Absent amino acids AGHMNPQTY

Common amino acids DW

Mass 2311.64

Pl 4.23

Basic residues 2

Acidic residues 4

Hydrophobic residues 9

Net charge -2

Boman Index -2527

Hydrophobicity -3.33

Aliphatic Index 102.78

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 16500

Absorbance 280nm 970.59

Polar residues 3

Literature Information

Literature 1

Title A virocidal amphipathic {alpha}-helical peptide that inhibits hepatitis C virus infection in vitro.

Pubmed ID 18287023

Reference Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3088-93.

Author Cheng G, Montero A, Gastaminza P, Whitten-Bauer C, Wieland SF, Isogawa M, Fredericksen B, Selvarajah S, Gallay PA, Ghadiri MR, Chisari FV.

DOI 10.1073/pnas.0712380105