General Information


DRAVP ID  DRAVPe01209

Peptide Name   RJ7

Sequence  AKKAWKKAKKAAKKAKKWAKK

Sequence Length  21

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  HSV

Assay  Plaque reduction assay,ELISA

Activity 

  • [Ref.15498607]herpes simplex virus 1(HSV-1):inhibition of HSV-1 infection in MRC-5 cells(IC50=40.8 μM);
  • herpes simplex virus 2(HSV-2):inhibition of HSV-2 infection in MRC-5 cells(IC50=44.9 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.15498607]human fibroblasts(MRC-5 cells):CC50>412 μM.

Binding Target  Not found

Mechanism  The peptide was able to inhibit the entry of HSV-1 into the host cell, probably by blocking heparan sulfate(HS) at the cell surface.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01209

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C115H201N35O22

Absent amino acids  CDEFGHILMNPQRSTVY

Common amino acids  K

Mass  2426.08

Pl  11.04

Basic residues  12

Acidic residues  0

Hydrophobic residues  9

Net charge  12

Boman Index  -4927

Hydrophobicity  -171.43

Aliphatic Index  33.33

Half Life 

  •     Mammalian:4.4 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  11000

Absorbance 280nm  550

Polar residues  0



Literature Information


Literature 1

Title   A wide range of medium-sized, highly cationic, alpha-helical peptides show antiviral activity against herpes simplex virus.

Pubmed ID   15498607

Reference   Antiviral Res. 2004 Nov;64(2):119-26.

Author   Jenssen H, Andersen JH, Mantzilas D, Gutteberg TJ. 

DOI   10.1016/j.antiviral.2004.08.003