General Information


DRAVP ID  DRAVPe01270

Peptide Name   HR2-18(HR2 region 1161–1187)

Sequence  IQKEIDRLNEVAKNLNESLIDLQELGK

Sequence Length  27

UniProt ID  P59594 

Source  Synthetic construct(derived from SARS-CoV spike protein)



Activity Information


Target Organism  SARS-CoV

Assay  luciferase assay

Activity 

  • [Ref.15184046]HIV-luc/SARS pseudotyped virus:inhibition of virus infection in 293T cells(EC50=1.19 μM);
  • SARS-CoV(wild-type):inhibition of virus infection in 293T cells(EC50=5.22 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  membrane

Mechanism  soluble synthesized HR1, HR2 or homologues can bind the exposed HR1 or HR2 region and block the formation of the six-helix bundle, thus inhibiting virus fusion with the target cell.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C135H232N38O46

Absent amino acids  CFHMPTWY

Common amino acids  L

Mass  3123.55

Pl  4.66

Basic residues  4

Acidic residues  6

Hydrophobic residues  10

Net charge  -2

Boman Index  -6450

Hydrophobicity  -64.44

Aliphatic Index  130

Half Life 

  •     Mammalian:20 hour
  •     Yeast:30 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  5



Literature Information


Literature 1

Title   Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein.

Pubmed ID   15184046

Reference   Biochem Biophys Res Commun. 2004 Jul 2;319(3):746-52.

Author   Yuan K, Yi L, Chen J, Qu X, Qing T, Rao X, Jiang P, Hu J, Xiong Z, Nie Y, Shi X, Wang W, Ling C, Yin X, Fan K, Lai L, Ding M, Deng H.

DOI   10.1016/j.bbrc.2004.05.046