General Information

General Information

DRAVP ID DRAVPe01303

Peptide Name Human defensin-5 (HD-5; Defensin, alpha 5; Human, mammals, animals)

Sequence ATCYCRTGRCATRESLSGVCEISGRLYRLCCR

Sequence Length 32

UniProt ID Q01523 A0JDY6 Q3KNV2

Source Homo sapiens (Human)

Activity Information

Target Organism SARS-CoV-2,HSV,HIV

Assay Plaque assay

Activity

[Ref.34206990]SARS-CoV-2:inhibition of infection in HEK293T-hACE2 cells(60% inhibition at 12.5 μg/mL (3.45 μM));
SARS-CoV-2 variant P.1:inhibition of infection in HeLa-hACE2 cells(72% inbibition at 50 μg/mL);
SARS-CoV-2 variant B.1.1.7:inhibition of infection in HeLa-hACE2 cells(32% inbibition at 50 μg/mL).
[Ref.23269800]Herpes simplex virus2(HSV-2):Potential for CaSki cell protection from HSV-2 infection(47.8% ±3.9% cell protection rates at 25 μg/ml,69.4% ± 5.4% cell protection at 50 μg/ml);neutralization activity against HSV-2 during the preinfection stage(IC50=35 μg/ml);
HIV-1:inhibition of virus infection in JLTRG cells(IC50=7.51 μg/ ml).

Hemolytic Activity

[Ref.26206286] It has 0% hemolysis at 100μM against human red blood cells.

Cytotoxicity

[Ref.34206990]No cytotoxicity on HEK293T cells up to 50 μg/mL.
[Ref.23269800]No cytotoxicity against CaSki cells up to 100μg/ ml.

Binding Target Not found

Mechanism The peptide exhibits antiviral activity by inhibiting virus entry.

Structure Information

PDB ID 1ZMP

Predicted Structure Download DRAVPe01303

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Free

Other Modification Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry L

Physicochemical Information

Formula C144H244N50O45S6

Absent amino acids DFHKMNPQW

Common amino acids CR

Mass 3588.19

Pl 8.96

Basic residues 6

Acidic residues 2

Hydrophobic residues 7

Net charge 4

Boman Index -8349

Hydrophobicity -11.25

Aliphatic Index 64.06

Half Life

Mammalian:4.4 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 3355

Absorbance 280nm 108.23

Polar residues 17

Literature Information

Literature 1

Title Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry.

Pubmed ID 34206990

Reference Viruses. 2021 Jun 26;13(7):1246.

Author Xu C, Wang A, Marin M, Honnen W, Ramasamy S, Porter E, Subbian S, Pinter A, Melikyan GB, Lu W, Chang TL.

DOI 10.3390/v13071246

Literature 2

Title Antimicrobial activity of human -defensin 5 and its linear analogs: N-terminal fatty acylation results in enhanced antimicrobial activity of the linear analogs.

Pubmed ID 26206286

Reference Peptides. 2015 Sep;71:128-40.

Author Basil Mathew Ramakrishnan Nagaraj

DOI 10.1016/j.peptides.2015.07.009

Literature 3

Title Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites.

Pubmed ID 23269800

Reference J Virol. 2013 Mar;87(5):2835-45.

Author Wang A, Chen F, Wang Y, Shen M, Xu Y, Hu J, Wang S, Geng F, Wang C, Ran X, Su Y, Cheng T, Wang J.

DOI 10.1128/JVI.02209-12