DRAVP ID  DRAVPe01303

Peptide Name   Human defensin-5 (HD-5; Defensin, alpha 5; Human, mammals, animals)

Sequence  ATCYCRTGRCATRESLSGVCEISGRLYRLCCR

Sequence Length  32

UniProt ID  Q01523  A0JDY6  Q3KNV2 

Taxon ID  None

Source  Homo sapiens (Human)

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  SARS-CoV-2,HSV,HIV

Assay  Plaque assay

Activity 

• [Ref.34206990]SARS-CoV-2:inhibition of infection in HEK293T-hACE2 cells(60% inhibition at 12.5 μg/mL (3.45 μM));
• SARS-CoV-2 variant P.1:inhibition of infection in HeLa-hACE2 cells(72% inbibition at 50 μg/mL);
• SARS-CoV-2 variant B.1.1.7:inhibition of infection in HeLa-hACE2 cells(32% inbibition at 50 μg/mL).
• [Ref.23269800]Herpes simplex virus2(HSV-2):Potential for CaSki cell protection from HSV-2 infection(47.8% ±3.9% cell protection rates at 25 μg/ml,69.4% ± 5.4% cell protection at 50 μg/ml);neutralization activity against HSV-2 during the preinfection stage(IC50=35 μg/ml);
• HIV-1:inhibition of virus infection in JLTRG cells(IC50=7.51 μg/ ml).

Hemolytic Activity 

• [Ref.26206286] It has 0% hemolysis at 100μM against human red blood cells.

Cytotoxicity 

• [Ref.34206990]No cytotoxicity on HEK293T cells up to 50 μg/mL.
• [Ref.23269800]No cytotoxicity against CaSki cells up to 100μg/ ml.

Binding Target  Not found

Mechanism  The peptide exhibits antiviral activity by inhibiting virus entry.

PDB ID  1ZMP 

Predicted Structure Download  DRAVPe01303

Linear/Cyclic  Cyclic

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry  L

Literature 1

Title   Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry.

Pubmed ID   34206990

Reference   Viruses. 2021 Jun 26;13(7):1246.

Author   Xu C, Wang A, Marin M, Honnen W, Ramasamy S, Porter E, Subbian S, Pinter A, Melikyan GB, Lu W, Chang TL.

DOI   10.3390/v13071246

Literature 2

Title   Antimicrobial activity of human -defensin 5 and its linear analogs: N-terminal fatty acylation results in enhanced antimicrobial activity of the linear analogs.

Pubmed ID   26206286

Reference   Peptides. 2015 Sep;71:128-40.

Author   Basil Mathew Ramakrishnan Nagaraj

DOI   10.1016/j.peptides.2015.07.009

Literature 3

Title   Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites.

Pubmed ID   23269800

Reference   J Virol. 2013 Mar;87(5):2835-45.

Author   Wang A, Chen F, Wang Y, Shen M, Xu Y, Hu J, Wang S, Geng F, Wang C, Ran X, Su Y, Cheng T, Wang J.

DOI   10.1128/JVI.02209-12