General Information

General Information

DRAVP ID DRAVPe01305

Peptide Name HD5[S23R]

Sequence ATCYCRTGRCATRESLSGVCEIRGRLYRLCCR

Sequence Length 32

UniProt ID Q01523

Source Synthetic construct(derived from human alpha defensin(HD5))

Activity Information

Target Organism HSV

Assay Plaque assay

Activity

[Ref.23269800]Herpes simplex virus2(HSV-2):neutralization activity against HSV-2 during the preinfection stage(IC50=36 μg/ml);

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.23269800]No cytotoxicity against CaSki cells up to 100μg/ ml.

Binding Target capsid protein gD

Mechanism The interaction between peptide and viral capsid proteins may cause aggregation of virions to prevent viruses from normal binding to target cells. Another possibility is that the binding of HD5 derivatives to the virion envelope blocks the epitopes of interaction between viruses and cell receptors, which are necessary for viral adsorption or entry into host cells.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01305

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Free

Other Modification Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry L

Physicochemical Information

Formula C147H251N53O44S6

Absent amino acids DFHKMNPQW

Common amino acids R

Mass 3657.3

Pl 9.22

Basic residues 7

Acidic residues 2

Hydrophobic residues 7

Net charge 5

Boman Index -9501

Hydrophobicity -22.81

Aliphatic Index 64.06

Half Life

Mammalian:4.4 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 3355

Absorbance 280nm 108.23

Polar residues 16

Literature Information

Literature 1

Title Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites.

Pubmed ID 23269800

Reference J Virol. 2013 Mar;87(5):2835-45.

Author Wang A, Chen F, Wang Y, Shen M, Xu Y, Hu J, Wang S, Geng F, Wang C, Ran X, Su Y, Cheng T, Wang J.

DOI 10.1128/JVI.02209-12