General Information


DRAVP ID  DRAVPe01526

Peptide Name   RatNP-1

Sequence  VTCYCRRTRCGFRERLSGACGYRGRIYRLCCR

Sequence Length  32

UniProt ID  Q62716 

Source  Rattus norvegicus



Activity Information


Target Organism  HSV

Assay 

Activity 

  • [Ref.12543649]HSV-2:inhibition of HSV-2 infection in Vero cells(98.7%±0.7% inhibition at 25 μg/ ml).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.12543649]No cytotoxic effect on CaSki cells was observed at the concentration of 25 or 100 μg/ml.

Binding Target  membrane

Mechanism  NP-1 inactivates HSV and prevents viral entry and cell-to-cell spread. NP-1 blocks fusion events important in HSV acquisition and transmission.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01526

Linear/Cyclic  Cyclic

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry  L



Physicochemical Information


Formula  C158H261N59O41S6

Absent amino acids  DHKMNPQW

Common amino acids  R

Mass  3835.54

Pl  9.99

Basic residues  9

Acidic residues  1

Hydrophobic residues  6

Net charge  8

Boman Index  -11502

Hydrophobicity  -49.38

Aliphatic Index  48.75

Half Life 

  •     Mammalian:100 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  4845

Absorbance 280nm  156.29

Polar residues  16



Literature Information


Literature 1

Title   NP-1, a rabbit alpha-defensin, prevents the entry and intercellular spread of herpes simplex virus type 2. 

Pubmed ID   12543649

Reference   Antimicrob Agents Chemother. 2003 Feb;47(2):494-500.

Author   Sinha S, Cheshenko N, Lehrer RI, Herold BC.

DOI   10.1128/AAC.47.2.494-500.2003