General Information

General Information

DRAVP ID DRAVPe01526

Peptide Name RatNP-1

Sequence VTCYCRRTRCGFRERLSGACGYRGRIYRLCCR

Sequence Length 32

UniProt ID Q62716

Source Rattus norvegicus

Activity Information

Target Organism HSV

Assay

Activity

[Ref.12543649]HSV-2:inhibition of HSV-2 infection in Vero cells(98.7%±0.7% inhibition at 25 μg/ ml).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.12543649]No cytotoxic effect on CaSki cells was observed at the concentration of 25 or 100 μg/ml.

Binding Target membrane

Mechanism NP-1 inactivates HSV and prevents viral entry and cell-to-cell spread. NP-1 blocks fusion events important in HSV acquisition and transmission.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01526

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Free

Other Modification Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry L

Physicochemical Information

Formula C158H261N59O41S6

Absent amino acids DHKMNPQW

Common amino acids R

Mass 3835.54

Pl 9.99

Basic residues 9

Acidic residues 1

Hydrophobic residues 6

Net charge 8

Boman Index -11502

Hydrophobicity -49.38

Aliphatic Index 48.75

Half Life

Mammalian:100 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 4845

Absorbance 280nm 156.29

Polar residues 16

Literature Information

Literature 1

Title NP-1, a rabbit alpha-defensin, prevents the entry and intercellular spread of herpes simplex virus type 2.

Pubmed ID 12543649

Reference Antimicrob Agents Chemother. 2003 Feb;47(2):494-500.

Author Sinha S, Cheshenko N, Lehrer RI, Herold BC.

DOI 10.1128/AAC.47.2.494-500.2003