General Information

General Information

DRAVP ID DRAVPe01588

Peptide Name NS5A-derived peptide C5A [I6L,I10L]

Sequence SWLRDLWDWLCEVLSDFK

Sequence Length 18

UniProt ID V5RF15

Source Synthetic construct

Activity Information

Target Organism HCV,HIV

Assay Pseudotyped HIV (HIVpp) and antiviral assay,HCV production and infection assay

Activity

[Ref.21801309]HCV: inhibition of HCVcc infection in Huh7.5.1 cells(IC50=1.40±0.15 μM);
HIV-1:inhibition of pseudotyped HIV infection in TZM-BL cells(IC50>5.00 μM).

Hemolytic Activity

[Ref.21801309]Human erythrocytes:MHC=6.92 μM.

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target membrane

Mechanism The peptide inhibits HCV and HIV infection in vitro, which can disrupt the integrity of the viral membrane and capsid core while preserving the integrity of host membranes.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01588

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C109H155N25O29S

Absent amino acids AGHIMNPQTY

Common amino acids L

Mass 2311.64

Pl 4.23

Basic residues 2

Acidic residues 4

Hydrophobic residues 9

Net charge -2

Boman Index -2527

Hydrophobicity -11.11

Aliphatic Index 102.78

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 16500

Absorbance 280nm 970.59

Polar residues 3

Literature Information

Literature 1

Title Rational design of peptides with anti-HCV/HIV activities and enhanced specificity.

Pubmed ID 21801309

Reference Chem Biol Drug Des. 2011 Nov;78(5):835-43.

Author Li GR, He LY, Liu XY, Liu AP, Huang YB, Qiu C, Zhang XY, Xu JQ, Yang W, Chen YX.

DOI 10.1111/j.1747-0285.2011.01201.x