General Information


DRAVP ID  DRAVPe01595

Peptide Name   NS5A-derived peptide C5A [I10S]

Sequence  SWLRDIWDWSCEVLSDFK

Sequence Length  18

UniProt ID  V5RF15 

Source  Synthetic construct



Activity Information


Target Organism  HCV,HIV

Assay  Pseudotyped HIV (HIVpp) and antiviral assay,HCV production and infection assay

Activity 

  • [Ref.21801309]HCV: inhibition of HCVcc infection in Huh7.5.1 cells(IC50>5.00 μM);
  • HIV-1:inhibition of pseudotyped HIV infection in TZM-BL cells(IC50>5.00 μM).

Hemolytic Activity 

  • [Ref.21801309]Human erythrocytes:MHC=28.00 μM.

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  membrane

Mechanism  The peptide inhibits HCV and HIV infection in vitro, which can disrupt the integrity of the viral membrane and capsid core while preserving the integrity of host membranes.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01595

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C106H149N25O30S

Absent amino acids  AGHMNPQTY

Common amino acids  DSW

Mass  2285.56

Pl  4.23

Basic residues  2

Acidic residues  4

Hydrophobic residues  8

Net charge  -2

Boman Index  -3359

Hydrophobicity  -32.78

Aliphatic Index  81.11

Half Life 

  •     Mammalian:1.9 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  16500

Absorbance 280nm  970.59

Polar residues  4



Literature Information


Literature 1

Title   Rational design of peptides with anti-HCV/HIV activities and enhanced specificity.

Pubmed ID   21801309

Reference   Chem Biol Drug Des. 2011 Nov;78(5):835-43.

Author   Li GR, He LY, Liu XY, Liu AP, Huang YB, Qiu C, Zhang XY, Xu JQ, Yang W, Chen YX.

DOI   10.1111/j.1747-0285.2011.01201.x