General Information

General Information

DRAVP ID DRAVPe01617

Peptide Name ApoEdpL-W

Sequence WRKWRKRWWWRKWRKRWW

Sequence Length 18

UniProt ID No entry found

Source Synthetic construct

Activity Information

Target Organism HSV,HIV,VSV

Assay plaque reduction assay

Activity

[Ref.17681018]HSV-1:inhibition of virus infection in NP-2/CD4/CCR5 cells(IC50=3.1 µM);
HIV-1:inhibition of virus replication(>80% inhibition at 10 µM).

Hemolytic Activity

[Ref.17681018]15% hemolysis against human red blood cells at 35 μM.

Cytotoxicity

[Ref.17681018]No significant cytotoxicity against Vero cells up to 40 μM.

Binding Target Not found

Mechanism The anti-HSV1 activity of apoEdp involves inhibition of virus particle attachment to cells, with this likely being related to the derivation of this peptide from the apoE HSPG/LDLR binding region.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01617

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C148H202N48O19

Absent amino acids ACDEFGHILMNPQSTVY

Common amino acids W

Mass 2957.54

Pl 12.7

Basic residues 10

Acidic residues 0

Hydrophobic residues 8

Net charge 10

Boman Index -9308

Hydrophobicity -276.67

Aliphatic Index 0

Half Life

Mammalian:2.8 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 44000

Absorbance 280nm 2588.24

Polar residues 0

Literature Information

Literature 1

Title Apolipoprotein E-derived antimicrobial peptide analogues with altered membrane affinity and increased potency and breadth of activity.

Pubmed ID 17681018

Reference FEBS J. 2007 Sep;274(17):4511-25.

Author Kelly BA, Neil SJ, McKnight A, Santos JM, Sinnis P, Jack ER, Middleton DA, Dobson CB.

DOI 10.1111/j.1742-4658.2007.05981.x