DRAVP ID  DRAVPe01617

Peptide Name   ApoEdpL-W

Sequence  WRKWRKRWWWRKWRKRWW

Sequence Length  18

UniProt ID  No entry found

Taxon ID  None

Source  Synthetic construct

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  HSV,HIV,VSV

Assay  plaque reduction assay

Activity 

• [Ref.17681018]HSV-1:inhibition of virus infection in NP-2/CD4/CCR5 cells(IC50=3.1 µM);
• HIV-1:inhibition of virus replication(>80% inhibition at 10 µM).

Hemolytic Activity 

• [Ref.17681018]15% hemolysis against human red blood cells at 35 μM.

Cytotoxicity 

• [Ref.17681018]No significant cytotoxicity against Vero cells up to 40 μM.

Binding Target  Not found

Mechanism  The anti-HSV1 activity of apoEdp involves inhibition of virus particle attachment to cells, with this likely being related to the derivation of this peptide from the apoE HSPG/LDLR binding region.

PDB ID  None

Predicted Structure Download  DRAVPe01617

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   Apolipoprotein E-derived antimicrobial peptide analogues with altered membrane affinity and increased potency and breadth of activity.

Pubmed ID   17681018

Reference   FEBS J. 2007 Sep;274(17):4511-25.

Author   Kelly BA, Neil SJ, McKnight A, Santos JM, Sinnis P, Jack ER, Middleton DA, Dobson CB.

DOI   10.1111/j.1742-4658.2007.05981.x