General Information


DRAVP ID  DRAVPe01638

Peptide Name   DENV Envelope glycoprotein (98-109)

Sequence  DRGWGNGCGLFG

Sequence Length  12

UniProt ID  Q5UB51  P17763 

Source  Synthetic construct(derived from DENV Envelope glycoprotein)



Activity Information


Target Organism  DENV

Assay  Focus-forming assay (FFA)

Activity 

  • [Ref.30508603]DENV-1:inhibition of virus infection in Vero cells(88%±3.89% inhibition at 50 μM,IC50=10 μM);
  • DENV-2:inhibition of virus infection in Vero cells(IC50=10 μM);
  • DENV-3:inhibition of virus infection in Vero cells(60% inhibition at 20μM);
  • DENV-4:inhibition of virus infection in Vero cells(55% inhibition at 20μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.30508603]<20% cytotoxicity against Vero and C6/36 cells at 200 μM.

Binding Target  E protein

Mechanism  The peptide acts as inhibitors by binding to the E protein, suppressing its conformational changes, and preventing its binding to the cellular receptor, thereby inhibiting viral entry.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C53H75N17O16S

Absent amino acids  AEHIKMPQSTVY

Common amino acids  G

Mass  1238.34

Pl  5.83

Basic residues  1

Acidic residues  1

Hydrophobic residues  3

Net charge  0

Boman Index  -1407

Hydrophobicity  -44.17

Aliphatic Index  32.5

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  5500

Absorbance 280nm  500

Polar residues  7



Literature Information


Literature 1

Title   In vitro analysis of synthetic peptides in blocking the entry of dengue virus.

Pubmed ID   30508603

Reference   Virus Res. 2019 Jan 15;260:142-150.

Author   John AM, Jittmittraphap A, Chattanadee S, Alwin Prem Anand A, Shenbagarathai R, Leaungwutiwong P.

DOI   10.1016/j.virusres.2018.11.016