General Information

General Information

DRAVP ID DRAVPe01744

Peptide Name MHV-S (1216-1254)

Sequence DLSLDFEKLNVTLLDLTYEMNRIQDAIKKLNESYINLKE

Sequence Length 39

UniProt ID P11224

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism SARS-CoV, MHV

Assay Infection inhibition assay

Activity

[Ref.15150417]SARS-CoV: inhibition of SARS-CoV infection in Vero 118 cells (EC50>50µM);
MHV(mouse hepatitis virus): inhibition of MHV infection in Vero 118 cells (EC50=0.9±0.1µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target membrane

Mechanism Inhibits virus infection by interfering with six-helix bundle formation(fusion inhibitor).

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C207H337N51O67S

Absent amino acids CGHPW

Common amino acids L

Mass 4644.31

Pl 4.51

Basic residues 5

Acidic residues 8

Hydrophobic residues 14

Net charge -3

Boman Index -7826

Hydrophobicity -42.56

Aliphatic Index 120

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:>10 hour

Extinction Coefficient cystines 2980

Absorbance 280nm 78.42

Polar residues 10

Literature Information

Literature 1

Title Severe acute respiratory syndrome coronavirus (SARS-CoV) infection inhibition using spike protein heptad repeat-derived peptides.

Pubmed ID 15150417

Reference Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8455-60.

Author Bosch BJ, Martina BE, Van Der Zee R, Lepault J, Haijema BJ, Versluis C, Heck AJ, De Groot R, Osterhaus AD, Rottier PJ.

DOI 10.1073/pnas.0400576101