General Information


DRAVP ID  DRAVPe01758

Peptide Name   DENV2 Ep (419-447)

Sequence  AWDFGSLGGVFTSIGKALHQVFGAIYGAA

Sequence Length  29

UniProt ID  Q9WDA6 

Source  Synthetic construct



Activity Information


Target Organism  DENV-2

Assay  Plaque assay

Activity 

  • [Ref.20881042]DENV2(dengue virus type 2): Inhibition of DENV-2 infection in BHK-21 cells (IC90~250µM)

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  low-pH endosome

Mechanism  an initial, nonspecific interaction of the hydrophobicC terminus of the peptide inhibitor with the viral membranecarries it with the virion into the endosome, allowing aspecific interaction of the peptide with domain II as theprotein reconfigures, inhibiting the final zipping of thestem



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C139H202N34O37

Absent amino acids  CEMNPR

Common amino acids  G

Mass  2941.34

Pl  6.79

Basic residues  2

Acidic residues  1

Hydrophobic residues  15

Net charge  1

Boman Index  1974

Hydrophobicity  73.79

Aliphatic Index  91.03

Half Life 

  •     Mammalian:4.4 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  6990

Absorbance 280nm  249.64

Polar residues  10



Literature Information


Literature 1

Title   Peptide inhibitors of flavivirus entry derived from the E protein stem.

Pubmed ID   20881042

Reference   J Virol. 2010 Dec;84(24):12549-54.

Author   Schmidt AG, Yang PL, Harrison SC.

DOI   10.1128/JVI.01440-10