General Information

General Information

DRAVP ID DRAVPe01769

Peptide Name CMK

Sequence RVKR

Sequence Length 4

UniProt ID No entry found

Source Synthetic construct

Activity Information

Target Organism SARS-CoV-2

Assay Plaque assay

Activity

[Ref.33007239]SARS-CoV-2:inhibition of virus production in Vero E6 cells(IC50=0.057 µM).
[Ref.31683742]Virus:Zika virus (ZIKV):inhibition of virus release in Vero cells(IC50=18.59 µM);Japanese encephalitis virus (JEV):inhibition of virus release in BHK-21 cells(IC50=19.91 µM).
[Ref.23617302]Hepatitis B virus (HBV):Inhibition of HBeAg secretion in HepG2.2.15 cells(26±11% inhibition at 20 µM,21±13% Inhibition at 100 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.31683742]Vero cells:CC50=712.9 µM.
[Ref.33007239]Vero E6 cells:IC50=318.2 µM.

Binding Target Not found

Mechanism CMK blocks virus entry, but it further suppresses cleavage of spikes and the syncytium.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Decanoyl(C10)

C-terminal Modification chloromethylketone(CMK)

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C23H47N11O5

Absent amino acids ACDEFGHILMNPQSTWY

Common amino acids R

Mass 557.7

Pl 12.01

Basic residues 3

Acidic residues 0

Hydrophobic residues 1

Net charge 3

Boman Index -3135

Hydrophobicity -217.5

Aliphatic Index 72.5

Half Life

Mammalian:1 hour
Yeast:2 min
E.coli:2 min

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 0

Literature Information

Literature 1

Title Therapeutic potential of furin inhibitors for the chronic infection of hepatitis B virus.

Pubmed ID 23617302

Reference Liver Int. 2013 Sep;33(8):1230-8.

Author Pang YJ, Tan XJ, Li DM, Zheng ZH, Lei RX, Peng XM.

DOI 10.1111/liv.12185

Literature 2

Title Decanoyl-Arg-Val-Lys-Arg-Chloromethylketone: An Antiviral Compound That Acts against Flaviviruses through the Inhibition of Furin-Mediated prM Cleavage.

Pubmed ID 31683742

Reference Viruses. 2019 Oct 31;11(11):1011.

Author Imran M, Saleemi MK, Chen Z, Wang X, Zhou D, Li Y, Zhao Z, Zheng B, Li Q, Cao S, Ye J.

DOI 10.3390/v11111011

Literature 3

Title Furin Inhibitors Block SARS-CoV-2 Spike Protein Cleavage to Suppress Virus Production and Cytopathic Effects.

Pubmed ID 33007239

Reference Cell Rep. 2020 Oct 13;33(2):108254.

Author Cheng YW, Chao TL, Li CL, Chiu MF, Kao HC, Wang SH, Pang YH, Lin CH, Tsai YM, Lee WH, Tao MH, Ho TC, Wu PY, Jang LT, Chen PJ, Chang SY, Yeh SH.

DOI 10.1016/j.celrep.2020.108254