General Information


DRAVP ID  DRAVPe01775

Peptide Name   P10

Sequence  SALEEQYKTFLDKFMHELEDLLYQLAL

Sequence Length  27

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  SARS-CoV-2

Assay  plaque assay

Activity 

  • [Ref.33580154]SARS-CoV-2:95% inhibition of replication in Vero-E6 cells at 10 μM;inhibition of replication in Calu-3 cells(IC50=42 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.33580154]The peptide proved to be devoid of cell toxicity on Vero-E6 and Calu-3 cells.

Binding Target  Not found

Mechanism  The peptide was designed mimicking the N-terminal helix of hACE2 protein,which could prevent the SARS-CoV-2 from infecting human cells, blocking the interaction between hACE2 and the virus spike protein.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C152H231N33O46S

Absent amino acids  CGINPRVW

Common amino acids  L

Mass  3288.76

Pl  4.35

Basic residues  3

Acidic residues  6

Hydrophobic residues  11

Net charge  -3

Boman Index  -3140

Hydrophobicity  -20

Aliphatic Index  108.52

Half Life 

  •     Mammalian:1.9 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  2980

Absorbance 280nm  114.62

Polar residues  4



Literature Information


Literature 1

Title   Human ACE2 peptide-mimics block SARS-CoV-2 pulmonary cells infection.

Pubmed ID   33580154

Reference   Commun Biol. 2021 Feb 12;4(1):197.

Author   Karoyan P, Vieillard V, Gómez-Morales L, Odile E, Guihot A, Luyt CE, Denis A, Grondin P, Lequin O.

DOI   10.1038/s42003-021-01736-8